New gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts.
New gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts.
复制标题
与吸血水蛭相关的新γ变形菌和水蛭内共生体的广泛系统发育分析。
DOI：
10.1128/aem.71.9.5219-5224.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Siddall,MarkE
中科院分区：
文献类型：
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作者：
Perkins,SusanL;Budinoff,RebeccaB;Siddall,MarkE
许多单食动物与细菌有共同进化的关系，为宿主提供不可用的营养。通常，这些微生物伴侣是垂直遗传的，居住在专门的结构或组织中。在这里，我们报告了吸血水蛭的三个新的细菌共生体谱系，一个来自亚马逊巨型水蛭，Haementeria ghilianii，另外两个来自placobdelloides物种。这些宿主各自具有不同的菌体或食管器官形态，其中细菌细胞所在。细菌16S rRNA基因的DNA测序和荧光原位杂交将这些共生体置于γ变形菌纲的两个独立分支中。我们还对本文报道的DNA序列以及文献中其他地方报道的细菌共生体进行了广泛的系统发育分析，包括水蛭属的甲变形菌共生体placobdellaas以及药用水蛭、Hirudo medicinalis和aRickettsiasp的veroni气单胞菌。在偏瘫边缘检出。综上所述，这些结果表明，吸血水蛭在其进化史上至少有五次与细菌结成伙伴关系。
Many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. Frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. Here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant Amazonian leech,Haementeria ghilianii, and two others fromPlacobdelloidesspecies. These hosts each possess a different mycetome or esophageal organ morphology where the bacterial cells are located. DNA sequencing of the bacterial 16S rRNA genes and fluorescent in situ hybridization placed these symbionts in two separate clades in the classGammaproteobacteria. We also conducted a broad phylogenetic analysis of the herein-reported DNA sequences as well as others from bacterial symbionts reported elsewhere in the literature, including alphaproteobacterial symbionts from the leech genusPlacobdellaas well asAeromonas veroniifrom the medicinal leech,Hirudo medicinalis, and aRickettsiasp. detected inHemiclepsis marginata. Combined, these results indicate that blood-feeding leeches have forged bacterial partnerships at least five times during their evolutionary history.