INHIBITION OF VIRUS-REPLICATION IN THE TONSILS OF PIGS PREVIOUSLY VACCINATED WITH A CHINESE STRAIN VACCINE AND CHALLENGED ORONASALLY WITH A VIRULENT-STRAIN OF CLASSICAL SWINE FEVER VIRUS
INHIBITION OF VIRUS-REPLICATION IN THE TONSILS OF PIGS PREVIOUSLY VACCINATED WITH A CHINESE STRAIN VACCINE AND CHALLENGED ORONASALLY WITH A VIRULENT-STRAIN OF CLASSICAL SWINE FEVER VIRUS
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DOI：
10.1016/0378-1135(87)90002-2
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发表时间：
1987-06-01
影响因子：
3.3
通讯作者：
VANDEPUTTE, J
中科院分区：
文献类型：
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作者：
BIRONT, P;LEUNEN, J;VANDEPUTTE, J
经口鼻感染经典猪瘟（C.S.F.）病毒后，从感染后第2天（p.i.）到死亡，在扁桃体中可检测到病毒增殖。在母体抗体存在或不存在的情况下，使用直接免疫荧光技术在低温切片和病毒分离上研究了先前接种过中国菌株疫苗的猪在o.n攻毒暴露后前10天的病毒复制过程。当仔猪在母体抗体存在的情况下被攻毒时，病毒在扁桃体中的复制仍然发生。而病毒的增殖周期和定位与母源抗体水平直接相关。母源抗体水平似乎也对疫苗预防挑战病毒在扁桃体复制的有效性负责：在存在低母源抗体滴度的情况下接种疫苗完全抑制病毒复制；在母体抗体滴度较高的情况下接种疫苗，只会缩短非口服强毒病毒的增殖周期。在这两种情况下，动物在接种疫苗后1周受到挑战。血清阴性动物的疫苗接种导致在完全育成期间扁桃体中的病毒复制几乎完全抑制：低温切片显示20只动物中有3只病毒复制有限。在其中一种动物中，病毒复制可能是如此微不足道，以至于病毒分离仍然是阴性的。
After an oronasal (O.N.) infection with classical swine fever (C.S.F.) virus, virus multiplication can be detected in the tonsils from Day 2 post infection (p.i.) till death. The course of viral replication during the first 10 days after O.N. challenge exposure of pigs, previously vaccinated with a Chinese strain vaccine in the presence or absence of maternal antibodies, was studied using direct immunofluorescence techniques on cryostat sections and virus isolations. When piglets were challenged O.N. in the presence of maternal antibodies, virus replication in the tonsils still occurred. The multiplication period and the localization of the virus, however, were directly correlated to the maternal antibody levels. The maternal antibody level also seems responsible for the efficacy of the vaccination to prevent challenge virus replication in the tonsils: vaccination in the presence of low maternal antibody titers completely inhibited virus replication; vaccination in the presence of high maternal antibody titers only reduced the multiplication period of the O.N.-administered virulent virus. In both cases, animals were challenged 1 week post vaccination. Vaccination of seronegative animals resulted in an almost complete inhibition of the virus replication in the tonsils during a full fattening period: cryostat sections revealed a limited virus replication in three out of 20 animals. In one of these animals, virus replication was probably so negligible that virus isolation remained negative.