Dietary selenium influences pancreatic tissue levels of selenoprotein W in chickens.
Dietary selenium influences pancreatic tissue levels of selenoprotein W in chickens.
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DOI：
10.1016/j.jinorgbio.2011.05.022
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发表时间：
2011-09
影响因子：
3.9
通讯作者：
Rihua Wang;Bo Sun;Ziwei Zhang;Shu Li;Shiwen Xu
中科院分区：
文献类型：
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作者：
Rihua Wang;Bo Sun;Ziwei Zhang;Shu Li;Shiwen Xu
硒影响哺乳动物体内硒蛋白W(SelW)的水平。然而，关于SelW在鸟类胰腺组织中的表达模式，人们知之甚少。为探讨日粮硒水平对雏鸡胰腺组织SelW基因表达的影响，将1日龄雏鸡随机分为3组，每组6只。L组饲喂低硒基础日粮(含Se 0.033 mg/kg)，M、H组饲喂含Se 0.15 mg/kg或1.5 mg/kg(亚硫酸钠)的补硒日粮55d。分别于15、25、35、45、55日龄取胰腺组织，检测Se含量和SelW基因表达水平。在H组，SelW的mRNA水平显著升高(P<；0.05)。当饲喂低硒基础日粮时，采样期内SelW基因的丰度显著降低(P<；0.05)。在这项研究中，还检测了两种酶，即硒半胱氨酸-tRNA[Ser]第二合成酶(SECS)和硒磷酸合成酶1(SPS1)。补硒组大鼠两种因子的mRNA表达水平均略有升高，而低硒饮食可下调SECs的mRNA表达。这些数据表明，SelW在鸟类的胰腺组织中表达，SelW基因的转录对饲料中的Se非常敏感。在本研究中，Se对SECs的mRNA水平也有影响，但对SPS1的影响不大。
Selenium (Se) influences the levels of selenoprotein W (SelW) in mammals. However, little is known about the pattern of SelW expression in the pancreatic tissue of birds. To investigate the effects of dietary Se levels on the expression of SelW mRNA in the pancreatic tissue of birds, one-day-old chickens were randomly allocated to three groups. The L group was fed a basal diet deficient in Se (containing 0.033mg/kg Se); the M and H groups were fed Se-supplemented diets with either 0.15 or 1.5mg/kg Se, respectively (as sodium selenite) for 55days. The pancreatic tissue was collected and examined for Se content and mRNA levels of SelW at 15, 25, 35, 45 and 55days old. In the H group, a significant increase (P<0.05) in mRNA levels of SelW was observed. When the chickens were fed a Se-deficient basal diet, the abundance of SelW mRNA significantly decreased (P<0.05) during the sampling period. In this study, two enzymes were also examined, namely, selenocysteine-tRNA[Ser]Secsynthase (SecS) and selenophosphate synthetase 1 (SPS1). The mRNA levels of two factors were slightly enhanced in the Se-supplemented groups, and a Se-deficient diet down regulated the mRNA expression of SecS. These data indicate that SelW is expressed in the pancreatic tissue of birds and that the transcription of the SelW gene is very sensitive to dietary Se. Se also has an effect on the mRNA levels of SecS, but has a little effect on SPS1 in this study.