Deletion of sites for initiation of DNA synthesis in the origin of broad host-range plasmid R1162.
Deletion of sites for initiation of DNA synthesis in the origin of broad host-range plasmid R1162.
复制标题
删除了广泛宿主范围质粒 R1162 起始 DNA 合成的位点。
DOI：
10.1016/0022-2836(90)90286-u
复制
发表时间：
1990
影响因子：
5.6
通讯作者：
Meyer,RJ
中科院分区：
文献类型：
--
作者：
Zhou,HS;Meyer,RJ
广宿主范围质粒R1162的复制起点含有两个相对的DNA合成起始位点。这些位点中的任一个都可以从R1162质粒衍生物中缺失。然而，所得到的质粒是不稳定的，在细胞中维持在较低的拷贝数，并形成二聚体和质粒繁殖所需的其他重组体。如果它含有两个相对的起始位点，则完整起源的性质得以恢复;一个起始位点可以取代另一个起始位点，并且每个位点不需要处于其原始方向。总的来说，结果表明，合成的每条链的R1162 DNA是在一个单一的网站，并没有有效的系统，在复制叉的运输过程中启动滞后链合成。
The origin of replication of the broad host-range plasmid R1162 contains two, oppositely facing initiation sites for DNA synthesis. Either of these sites can be deleted from an R1162 plasmid derivative. However, the resulting plasmids are unstable, maintained at a lower copy-number in the cell, and form dimers and other recombinants that are required for propagation of the plasmid.In vitro, a derivative lacking one initiation site is deficient in synthesis of the strand normally initiated from that site. The properties of the intact origin are restored if it contains two oppositely facing sites; one initiation site may substitute for the other, and each site need not be in its original orientation. Overall, the results suggest that synthesis of each strand of R1162 DNA is initiated at a single site, and that there is no efficient system for initiation of lagging strand synthesis during transit of the replication forks.