The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events.
The Schizosaccharomyces pombe rec16 gene product regulates multiple meiotic events.
复制标题
粟酒裂殖酵母rec16 基因产物调节多个减数分裂事件。
DOI：
10.1093/genetics/146.1.57
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Smith,GR
中科院分区：
文献类型：
--
作者：
Li,YF;Smith,GR
Previously isolated meiotic recombination (rec) mutants ofSchizosaccharomyces pombedefine 16 complementation groups. Therecgenes cloned and sequenced to date reveal little amino acid sequence identity to other reported proteins. We examined therecmutants for alterations in meiotic events other than recombination to gain insight into therecgene functions and to assess whether they affect recombination directly or indirectly. While mutations in therec6–12, 14, 15and19genes appeared to affect only meiotic recombination, a mutation inrec16delayed meiotic DNA synthesis and, in some instances, reduced its amount; mitotic DNA synthesis was not detectably altered, indicating that therec16effect is limited to meiosis. In therec16mutant some meiotically induced transcripts (e.g., rec7and15) were significantly reduced in abundance, whereas others (e.g., rec10andexo1) were induced and degraded with normal timing and extent during meiosis, indicating that therec16mutation leaves the basic meiotic program intact. These results indicate that therecgenes other thanrec16have their primary effect on meiotic recombination. In contrast, therec16gene product is essential for normal meiotic replication, recombination, and induction of some transcripts. These meiotic events may be coupled via a dependence of recombination and transcription on replication or via a cascade of gene expression.
