Regulation of denitrification at the cellular level: a clue to the understanding of N2O emissions from soils.
Regulation of denitrification at the cellular level: a clue to the understanding of N2O emissions from soils.
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DOI：
10.1098/rstb.2011.0321
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发表时间：
2012-05-05
期刊：
影响因子：
--
通讯作者：
Frostegård A
中科院分区：
文献类型：
--
作者：
Bakken LR;Bergaust L;Liu B;Frostegård A
反硝化原核生物利用氮氧化物作为终端电子受体，以响应氧气消耗。该过程排放出NO、N2 O和N2的混合物，这取决于催化NO3-逐步还原为N2 O并最终还原为N2的酶的相对活性。在标准化条件下测试时，培养的反硝化原核生物在从好氧呼吸到缺氧呼吸的过渡过程中表现出NO2−、NO和N2 O的特征性瞬时积累，但这种特征似乎与繁殖无关。因此，尽管土壤的酸化群落在排放N2 O的倾向上可能不同，但通过分析群落组成可能难以预测这些特征。我们实验室测试的菌株的一个共同特征是产生的N2 O的相对量（N2 O/（N2+ N2 O）产物比）与酸度相关，这显然是由于N2 O还原酶的组装受到干扰。土壤和从土壤中提取的微生物群落也表现出同样的现象。施用石灰是减少N2 O排放的一种方法，但需要通过田间试验进行验证。随着我们对细胞水平上的反硝化调节了解的更多，未来可能会出现更复杂的减排方法。
Denitrifying prokaryotes use NOx as terminal electron acceptors in response to oxygen depletion. The process emits a mixture of NO, N2O and N2, depending on the relative activity of the enzymes catalysing the stepwise reduction of NO3− to N2O and finally to N2. Cultured denitrifying prokaryotes show characteristic transient accumulation of NO2−, NO and N2O during transition from oxic to anoxic respiration, when tested under standardized conditions, but this character appears unrelated to phylogeny. Thus, although the denitrifying community of soils may differ in their propensity to emit N2O, it may be difficult to predict such characteristics by analysis of the community composition. A common feature of strains tested in our laboratory is that the relative amounts of N2O produced (N2O/(N2+N2O) product ratio) is correlated with acidity, apparently owing to interference with the assembly of the enzyme N2O reductase. The same phenomenon was demonstrated for soils and microbial communities extracted from soils. Liming could be a way to reduce N2O emissions, but needs verification by field experiments. More sophisticated ways to reduce emissions may emerge in the future as we learn more about the regulation of denitrification at the cellular level.
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DOI：
10.1099/mic.0.054148-0
发表时间：
2012-03
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Bergaust L;van Spanning RJM;Frostegård Å;Bakken LR
通讯作者：
Bakken LR
影响因子：
11.4
作者：
Bergsma, TT;Ostrom, NE;Robertson, GP
通讯作者：
Robertson, GP
影响因子：
4.2
作者：
Braker, Gesche;Dorsch, Peter;Bakken, Lars R.
通讯作者：
Bakken, Lars R.
影响因子：
5.1
作者：
Bergaust, Linda;Shapleigh, James;Bakken, Lars
通讯作者：
Bakken, Lars
影响因子：
3.1
作者：
Frolking, SE;Mosier, AR;Smith, KA
通讯作者：
Smith, KA