A novel non-hydrolytic protein from Pseudomonas oryzihabitans enhances the enzymatic hydrolysis of cellulose.
A novel non-hydrolytic protein from Pseudomonas oryzihabitans enhances the enzymatic hydrolysis of cellulose.
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来自 Pseudomonas oryzihabitans 的新型非水解蛋白可增强纤维素的酶水解。
DOI：
10.1016/j.jbiotec.2013.07.028
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发表时间：
2013
影响因子：
4.1
通讯作者：
Aixing Tang
中科院分区：
文献类型：
--
作者：
Yi;Heng Tao;You;Yan;Jing;Aixing Tang
已知几种蛋白质如膨胀蛋白、膨胀蛋白样蛋白和LPMO（溶解性多糖单加氧酶）对纤维素酶活性发挥增强作用。本研究从稻栖假单胞菌（PseudomonasoryzihabitansCGMCC 6169）的发酵滤液中分离纯化了一种新型的纤维素酶增效蛋白POEP 1，经SDS-PAGE分析，其分子量为60 kDa。质谱分析表明，它是一个未知的蛋白质，与expansin和expansin-like蛋白没有序列相似性。滤纸的酶水解的评价显示，POEP 1没有纤维素酶活性，但在纤维素酶浓度为0.1 FPU/g滤纸时显示出364%的高协同活性。当每克纤维素含有0.6 FPU纤维素酶和700 μg POEP 1时，达到最大糖产率，其是单独纤维素酶的2.2倍。POEP 1与膨胀蛋白和膨胀蛋白样蛋白具有相似的功能，可降低氢键强度和结晶度，导致滤纸破裂。这项研究为自然界中存在与扩展蛋白和扩展蛋白样蛋白具有相同功能的新型细菌蛋白提供了证据。
Several kinds of protein such as the expansin, expansin-like proteins and LPMOs (lytic polysaccharide monooxygenases) are known to exert enhancement effects on cellulase activity. In this study, a novel cellulase synergistic protein named POEP1 was purified from the culture filtrate ofPseudomonas oryzihabitansCGMCC 6169, and was homogeneous on SDS-PAGE with a molecular weight of 60 kDa. Mass spectrometry analysis indicated that it was an unknown protein without sequence similarity to the expansin and expansin-like proteins. Evaluation of the enzymatic hydrolysis of filter paper revealed that POEP1 had no cellulase activity but displayed high synergistic activity of 364% at a cellulase concentration of 0.1 FPU/g of filter paper. When a mixture containing 0.6 FPU cellulase and 700 μg POEP1 per g of cellulose was evaluated, the maximal sugar yield was achieved, which was 2.2-fold greater than that with the cellulase alone. POEP1 was found to have functional similarity to the expansin and expansin-like proteins, which could decrease both the hydrogen-bond intensity and crystallinity, and cause the filter paper disruption. This study provided evidence for the existence of novel bacterial proteins in nature serving the same function as expansin and expansin-like proteins.
影响因子：
4.4
作者：
Chen, Xin-ai;Ishida, Nobuhiro;Kitamoto, Katsuhiko
通讯作者：
Kitamoto, Katsuhiko