IL-10 inhibits HIV-1 replication and is induced by tat.
IL-10 inhibits HIV-1 replication and is induced by tat.
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IL-10 抑制 HIV-1 复制并由 tat 诱导。
DOI：
10.1006/bbrc.1994.1938
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发表时间：
1994
影响因子：
3.1
通讯作者：
Gill,PS
中科院分区：
文献类型：
--
作者：
Masood,R;Lunardi-Iskandar,Y;Moudgil,T;Zhang,Y;Law,RE;Huang,CL;Puri,RK;Levine,AM;Gill,PS
白介素10(IL-10)由TH2淋巴细胞产生，对淋巴样细胞和髓系细胞均有调节作用。在本研究中，我们证明了IL-10在所有被测试的HIV-1感染者、19例HIV阴性淋巴瘤中的3例和5名健康对照中没有人的外周血单核细胞(PBMC)中自发地表达和产生。从HIV感染者PBMCs分离的单核/巨噬细胞和T淋巴细胞中均检测到IL-10mRNA。我们还发现，感染HIV的前单核细胞(U937)和T(H9)细胞株能刺激IL-10的分泌。此外，稳定转染HIV Tat表达载体的T细胞株(H9)可分泌较高水平的IL-10。我们还证明了rhIL-10以剂量依赖的方式抑制HIV-1在感染的单核细胞和PBMC中的复制。因此，IL-10可能参与了HIV-1感染和艾滋病发展之间的长时间潜伏期。
Interleukin 10 (IL-10) is produced by TH2 lymphocytes and regulates both lymphoid and myeloid cells. In the present study we demonstrate that IL-10 is expressed and produced spontaneously in the peripheral blood mononuclear cells (PBMCs) of all HIV-1 infected individuals tested, 3 of 19 cases of HIV-negative lymphoma and none of five healthy controls. IL-10 mRNA was detectable in both monocytes/macrophages and T lymphocytes isolated from PBMCs of HIV infected patients. We have also shown that infection of promonocytic (U937) and T (H9) cell lines with HIV stimulates IL-10 secretion. Furthermore, a T cell line (H9) stably transfected with a HIV tat expression-vector secreted higher levels of IL-10. We have also demonstrated that rhIL-10 inhibited HIV-1 replication in infected monocytes and PBMCs in a dose dependent manner. IL-10 may thus participate in long latency between HIV-1 infection and development of AIDS.