THE CHEMOLITHOTROPHIC AMMONIUM-OXIDIZING COMMUNITY IN A NITROGEN-SATURATED ACID FOREST SOIL IN RELATION TO PH-DEPENDENT NITRIFYING ACTIVITY
THE CHEMOLITHOTROPHIC AMMONIUM-OXIDIZING COMMUNITY IN A NITROGEN-SATURATED ACID FOREST SOIL IN RELATION TO PH-DEPENDENT NITRIFYING ACTIVITY
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DOI：
10.1016/0038-0717(92)90223-k
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发表时间：
1992-03-01
影响因子：
9.7
通讯作者：
LAANBROEK, HJ
中科院分区：
文献类型：
--
作者：
DEBOER, W;TIETEMA, A;LAANBROEK, HJ
研究了氮饱和酸性花旗松林土壤不同层pH与硝化作用的关系。地上降解针叶层、枯枝落叶层和发酵层的硝化潜力（以干重计）远高于腐殖质和上部矿物质层。在所有层中，硝酸盐的产生可能是由于化能营养细菌产生的，因为它受到乙炔的抑制。枯落物层含有相对较高数量（10（5）g干土-1）的酸敏感铵氧化细菌，而其他层中的数量仅高于或低于MPN方法的检测限（10（3）g干土-1）。对土壤悬浮液中硝酸盐产量的测量表明，pH 值增加（pH 6 与 pH 4）会刺激枯落物层中的铵氧化，但不会或在较小程度上刺激发酵层和腐殖质层中的铵氧化。有人认为，酸敏感和耐酸的铵氧化细菌都有助于凋落物层的硝化作用。在发酵层和腐殖质层中，耐酸甚至嗜酸细菌被认为是造成铵氧化的原因。
The relationship between pH and nitrification in different layers of a nitrogen-saturated acid Douglas fir forest soil was studied. Nitrification potentials (on basis of dry weight) of overground degrading needles, litter and fermentation layers were much higher than those of the humus and upper mineral layers. In all layers, nitrate production was probably due to chemolithotrophic bacteria as it was inhibited by acetylene. The litter layer contained relatively high numbers (10(5) g dry soil-1) of acid-sensitive ammonium-oxidizing bacteria, whereas the numbers in the other layers were just above or lower than the detection limit (10(3) g dry soil-1) of the MPN method used. Measurements of nitrate production in soil suspensions indicated that a pH increase (pH 6 vs pH 4) stimulated ammonium oxidation in the litter layer but not or to a lesser extent in the fermentation and humus layers. It is argued that both acid-sensitive and acid-tolerant ammonium-oxidizing bacteria are contributing to nitrification in the litter layer. In the fermentation and humus layers acid-tolerant or even acidophilic bacteria are thought to be responsible for ammonium oxidation.