Supraparticles comprised of molecularly imprinted nanoparticles and modified gold nanoparticles as a nanosensor platform
Supraparticles comprised of molecularly imprinted nanoparticles and modified gold nanoparticles as a nanosensor platform
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DOI：
10.1039/c3ra43660h
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发表时间：
2013-11
期刊：
影响因子：
3.9
通讯作者：
Akane Uchida;Yukiya Kitayama;Eri Takano;T. Ooya;T. Takeuchi
中科院分区：
文献类型：
--
作者：
Akane Uchida;Yukiya Kitayama;Eri Takano;T. Ooya;T. Takeuchi
提出了一种基于分子印迹聚合物纳米粒子（MIP-NPs）和修饰金纳米粒子（Au-NPs）的高选择性、高灵敏度的小分子纳米传感系统。MIP-NPs作为分子识别材料，Au-NPs作为信号传导材料，从基于分子识别的结合事件到Au-NPs在可见光区的局部表面等离子体共振（LSPR）的光谱变化。这种新型的基于超粒子的纳米传感系统能够实现对目标分子的特异性传感，可以简单，快速和廉价地实现，仅使用紫外可见分光光度计。此外，亲和常数是120 000倍以上，比以前报道的与此传感系统。结果，成功地检测到了亚纳摩尔浓度的靶分子，这与相应的酶联免疫吸附测定法相当。所提出的基于超粒子的光谱传感系统可以在广泛的重要研究/工业领域（如环境，生命，医学和制药科学）中为传感工具打开一个新时代。
A highly selective and sensitive nanosensing system for small molecules is proposed based on the supraparticles of molecularly imprinted polymer nanoparticles (MIP-NPs) and modified gold nanoparticles (Au-NPs). The MIP-NPs function as molecular recognition materials and the Au-NPs function as signal transduction materials, from molecular recognition-based binding events to spectral changes of localized surface plasmon resonance (LSPR) of Au-NPs in the visible region. This novel supraparticles-based nanosensing system enables specific sensing towards target molecules that can be achieved simply, rapidly and inexpensively, using only a UV-vis spectrophotometer. Moreover, an affinity constant is obtained with this sensing system that is 120 000 times larger than that previously reported. As a result, a sub-nanomolar concentration of target molecules was successfully detected, which is comparable to the corresponding enzyme-linked immunosorbent assays. The proposed supraparticles-based spectroscopic sensing system could open a new era for sensing tools in a broad range of important research/industrial fields, such as the environmental, life, medical and pharmaceutical sciences.