Tunability and noise dependence in differentiation dynamics
Tunability and noise dependence in differentiation dynamics
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DOI：
10.1126/science.1137455
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发表时间：
2007-03-23
期刊：
影响因子：
56.9
通讯作者：
Elowitz, Michael B.
中科院分区：
文献类型：
--
作者：
Sueel, Guerol M.;Kulkarni, Rajan P.;Elowitz, Michael B.
分化的动态过程取决于基础遗传电路的结构、定量参数和噪声。然而，目前还不清楚这些元素如何联合收割机控制细胞的行为。我们分析了枯草芽孢杆菌细胞分化为感受态的概率和瞬时分化。一些关键参数独立地调整启动频率和能力发作的持续时间，并允许电路访问不同的动态机制，包括振荡。改变电路结构表明，能力事件的持续时间可以更精确。我们使用了一种实验方法来减少整体细胞噪声，并表明噪声水平与分化事件的频率相关。总之，这些数据揭示了一个依赖于噪声的电路，它在动态行为方面具有显著的弹性和可调性。
The dynamic process of differentiation depends on the architecture, quantitative parameters, and noise of underlying genetic circuits. However, it remains unclear how these elements combine to control cellular behavior. We analyzed the probabilistic and transient differentiation of Bacillus subtilis cells into the state of competence. A few key parameters independently tuned the frequency of initiation and the duration of competence episodes and allowed the circuit to access different dynamic regimes, including oscillation. Altering circuit architecture showed that the duration of competence events can be made more precise. We used an experimental method to reduce global cellular noise and showed that noise levels are correlated with frequency of differentiation events. Together, the data reveal a noise-dependent circuit that is remarkably resilient and tunable in terms of its dynamic behavior.