Spontaneous production of immunoglobulin M in human epithelial cancer cells.
Spontaneous production of immunoglobulin M in human epithelial cancer cells.
复制标题
DOI：
10.1371/journal.pone.0051423
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Qiu X
中科院分区：
文献类型：
--
作者：
Hu F;Zhang L;Zheng J;Zhao L;Huang J;Shao W;Liao Q;Ma T;Geng L;Yin CC;Qiu X
众所周知，B-1B细胞是产生天然免疫球蛋白M(IgM)的主要细胞类型，可通过增加IgM的分泌来应对感染。然而，我们意外地发现，一些上皮细胞也可以表达重排的IgM转录本，具有自然的IgM特征，如种系编码和限制性重排模式。在这里，我们研究了人非B细胞中IgM的表达，发现许多人上皮性癌细胞以及非癌上皮细胞都经常表达IgM。此外，CD79A和CD79B这两个与B细胞表面膜上的IgM物理连接形成B细胞抗原受体复合体的分子也表达在上皮性癌细胞的细胞表面，并与IgM共定位。与天然的IgM一样，上皮性癌细胞来源的IgM识别一系列微生物抗原，如单链DNA、双链DNA、脂多糖和Hep-2细胞抗原。更重要的是，模拟细菌感染的Toll样受体9(TLR9)的刺激，大大增加了人类上皮性癌细胞中IgM的分泌。这些发现表明，人上皮癌细胞和非癌上皮细胞都能自发产生具有天然抗体活性的IgM。
It is well known that B-1 B cells are the main cell type that is responsible for the production of natural immunoglobulin M (IgM) and can respond to infection by increasing IgM secretion. However, we unexpectedly found that some epithelial cells also can express rearranged IgM transcript that has natural IgM characteristics, such as germline-encoded and restricted rearrangement patterns. Here we studied IgM expression in human non-B cells and found that IgM was frequently expressed by many human epithelial cancer cells as well as non-cancer epithelial cells. Moreover, CD79A and CD79B, two molecules that are physically linked to membranous IgM on the surface of B cells to form the B cell antigen receptor complex, were also expressed on the cell surface of epithelial cancer cells and co-located with IgM. Like the natural IgM, the epithelial cancer cell-derived IgM recognized a series of microbial antigens, such as single-stranded DNA, double-stranded DNA, lipopolysaccharide, and the HEp-2 cell antigen. More important, stimulation of the toll-like receptor 9 (TLR9), which mimics bacterial infection, substantially increased the secretion of IgM in human epithelial cancer cells. These findings indicate that human epithelial cancer cells as well as non-cancer epithelial cells can spontaneously produce IgM with natural antibody activity.
登录
查看更多内容
影响因子：
11.4
作者：
Jego, G;Bataille, R;Pellat-Deceunynck, C
通讯作者：
Pellat-Deceunynck, C
影响因子：
5.4
作者：
Billstrom, MA;Johnson, GL;Worthen, GS
通讯作者：
Worthen, GS
影响因子：
4.8
作者：
Liu, Hai-dan;Zheng, Hui;Cao, Ya
通讯作者：
Cao, Ya
影响因子：
5.8
作者：
Droemann D;Albrecht D;Gerdes J;Ulmer AJ;Branscheid D;Vollmer E;Dalhoff K;Zabel P;Goldmann T
通讯作者：
Goldmann T
DOI：
10.1179/096805104225006525
发表时间：
2004-01-01
期刊：
JOURNAL OF ENDOTOXIN RESEARCH
影响因子：
--
作者：
Latz, E;Visintin, A;Golenbock, DT
通讯作者：
Golenbock, DT