Identification of CXorf1, a novel intronless gene in Xq27.3, expressed in human hippocampus.
Identification of CXorf1, a novel intronless gene in Xq27.3, expressed in human hippocampus.
复制标题
CXorf1 的鉴定，CXorf1 是 Xq27.3 中的一种新型无内含子基因，在人海马中表达。
DOI：
10.1089/dna.1998.17.1009
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发表时间：
1998
影响因子：
3.1
通讯作者：
Zucchi,I
中科院分区：
文献类型：
--
作者：
Redolfi,E;Montagna,C;Mumm,S;Affer,M;Susani,L;Reinbold,R;Hol,F;Vezzoni,P;Cimino,M;Zucchi,I
我们已经确定并鉴定了一个新的人类基因（基因组数据库GDB命名委员会指定的符号CXorf 1），该基因定位于Xq 27中X染色体长臂的DXS 369和DXS 181位点之间，大约2.5 Mb位于FMR 1基因的着丝粒处。CXorf 1基因在灵长类动物、牛和马中是保守的，但在小鼠和大鼠中不存在。北方印迹分析显示两种转录本，分别存在于大脑和G361黑色素瘤细胞系中。在人类海马切片上进行的原位杂交实验显示，CXorf 1 mRNA在该脑区的特定亚区中存在清晰、不均匀的定位。特别是，CXorf 1定位于齿状回的颗粒细胞层和阿蒙角的CA 2-CA 3亚区。CXorf 1是该区域最早被详细表征的基因之一，并且根据其染色体位置和表达模式，可能在大脑中具有重要功能。
We have identified and characterized a novel human gene (Nomenclature Committee of the Genome Database GDB-assigned symbolCXorf1) that maps to the long arm of the X chromosome in Xq27 between loci DXS369 and DXS181, approximately 2.5 Mb centromeric to theFMR1gene. TheCXorf1gene is conserved in primates, cow, and horse but not in mouse and rat. Northern blot analysis revealed two transcripts, present in the brain and in the G361 melanoma cell line.In situhybridization experiments performed on sections of human hippocampus showed a clear, uneven localization of theCXorf1mRNA in specific subfields of this brain area. In particular,CXorf1was localized in the granular-cell layer of the dentate gyrus and in the CA2-CA3 subfields of Ammon's horn.CXorf1is one of the first genes from this region to be characterized in detail and, on the basis of its chromosomal location and expression pattern, may have an important function in the brain.