P53 FUNCTIONS AS A CELL-CYCLE CONTROL PROTEIN IN OSTEOSARCOMAS
P53 FUNCTIONS AS A CELL-CYCLE CONTROL PROTEIN IN OSTEOSARCOMAS
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DOI：
10.1128/mcb.10.11.5772
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发表时间：
1990-11-01
影响因子：
5.3
通讯作者：
FRIEND, SH
中科院分区：
文献类型：
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作者：
DILLER, L;KASSEL, J;FRIEND, SH
p53基因突变与包括骨肉瘤在内的多种人类肿瘤有关。虽然已经表明野生型p53可以阻断E1a和ras共转化原代啮齿动物细胞的能力，但人们对p53基因失活对肿瘤形成的重要性知之甚少。我们发现，过度表达的野生型p53基因编码的骨肉瘤细胞的生长受阻。确定生长停滞是由于转染的细胞不能进展到S期。这表明p53基因作为抗癌基因的作用可能是以类似于酿酒酵母中检查点控制基因的方式控制细胞周期。
Mutations in the p53 gene have been associated with a wide range of human tumors, including osteosarcomas. Although it has been shown that wild-type p53 can block the ability of E1a and ras to cotransform primary rodent cells, it is poorly understood why inactivation of the p53 gene is important for tumor formation. We show that overexpression of the gene encoding wild-type p53 blocks the growth of osteosarcoma cells. The growth arrest was determined to be due to an inability of the transfected cells to progress into S phase. This suggests that the role of the p53 gene as an antioncogene may be in controlling the cell cycle in a fashion analogous to the check-point control genes in Saccharomyces cerevisiae.