Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper.
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper.
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DOI：
10.1080/20013078.2017.1286095
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发表时间：
2017
影响因子：
16
通讯作者：
Nolte-'t Hoen EN
中科院分区：
文献类型：
--
作者：
Mateescu B;Kowal EJ;van Balkom BW;Bartel S;Bhattacharyya SN;Buzás EI;Buck AH;de Candia P;Chow FW;Das S;Driedonks TA;Fernández-Messina L;Haderk F;Hill AF;Jones JC;Van Keuren-Jensen KR;Lai CP;Lässer C;Liegro ID;Lunavat TR;Lorenowicz MJ;Maas SL;Mäger I;Mittelbrunn M;Momma S;Mukherjee K;Nawaz M;Pegtel DM;Pfaffl MW;Schiffelers RM;Tahara H;Théry C;Tosar JP;Wauben MH;Witwer KW;Nolte-'t Hoen EN
含有rna的细胞外囊泡（EV）释放到细胞外环境中已经在许多不同的体外细胞系统和各种体液中得到证实。含有rna的EV因其将遗传编码信息传递给其他细胞的能力、作为疾病候选生物标志物的适用性以及作为治疗剂的用途而备受关注。尽管EV-RNA引起了基础研究人员、临床医生和工业界的极大兴趣，但我们目前对驱动和调节RNA入EV的机制以及RNA编码信息如何影响EV靶向细胞的信号传导过程的知识有限。此外，EV-RNA研究面临着各种技术挑战，例如EV分离方法的标准化，EV中微量RNA分离和表征方法的优化，以及证明EV-RNA在体内功能转移的方法的开发。这些主题在2015年国际细胞外囊泡学会的EV-RNA研讨会上进行了讨论。这篇立场文件是由研讨会的参与者撰写的，不仅概述了该领域的知识现状，而且还澄清了我们对EV(- rna)s的性质以及如何有效可靠地研究它们的不完整知识，目前禁止在EV- rna研究中实施金标准。此外，本文还提出了目前用于研究EV-RNA的策略的可能性和局限性，并呼吁在解释所获得的数据时要谨慎。
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.