Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae
Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae
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DOI：
10.1039/c6md00603e
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发表时间：
2017-02-01
期刊：
影响因子：
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通讯作者：
Moir, James W. B.
中科院分区：
文献类型：
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作者：
Ward, Jonathan S.;Morgan, Rebecca;Moir, James W. B.
一氧化碳释放分子（CO-RMs）作为抗菌剂的潜力代表了对抗抗生素耐药性的令人兴奋的前景。色氨酸- corm是一种含有锰(I)羰基的色氨酸，在光激活后对大肠杆菌具有毒性。在这里，我们证明了在没有光激活的情况下，Trypto-CORM对淋病奈瑟菌具有毒性。高CO亲和力的腿血红蛋白（Leg-Hb）逆转了Trypto-CORM毒性，表明毒性是由于CO释放引起的。在体外用Leg-Hb（而不是肌红蛋白）也可以检测到黑暗中从Trypto-CORM中释放的CO。淋病奈瑟菌对co基毒性比其他模式细菌病原体更敏感，可能作为使用CO-RMs进行抗菌治疗的可行候选者。
The potential for carbon monoxide-releasing molecules (CO-RMs) as antimicrobials represents an exciting prospective in the fight against antibiotic resistance. Trypto-CORM, a tryptophan-containing manganese(I) carbonyl, is toxic against E. coli following photo-activation. Here, we demonstrate that Trypto-CORM is toxic against Neisseria gonorrhoeae in the absence of photoactivation. Trypto-CORM toxicity was reversed by the high CO affinity globin leg-haemoglobin (Leg-Hb), indicating that the toxicity is due to CO release. Release of CO from Trypto-CORM in the dark was also detected with Leg-Hb (but not myoglobin) in vitro. N. gonorrhoeae is more sensitive to CO-based toxicity than other model bacterial pathogens, and may serve as a viable candidate for antimicrobial therapy using CO-RMs.