Abundance and diversity of aerobic heterotrophic microorganisms and their interaction with cyanobacteria in the oxic layer of an intertidal hypersaline cyanobacterial mat
Abundance and diversity of aerobic heterotrophic microorganisms and their interaction with cyanobacteria in the oxic layer of an intertidal hypersaline cyanobacterial mat
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DOI：
10.1093/femsec/fix183
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发表时间：
2018-02-01
影响因子：
4.2
通讯作者：
de Beer, Dirk
中科院分区：
文献类型：
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作者：
Abed, Raeid M. M.;Kohls, Katharina;de Beer, Dirk
需氧异养微生物（AH）在蓝藻垫碳循环中起着重要作用；然而，人们对它们的丰度、多样性和与蓝藻的相互作用知之甚少。使用催化报告沉积荧光原位杂交（CARD-FISH），毡层氧层细菌计数平均达到2.23 +/- 0.4 x 10（10）个细胞g（-1）。菌株分别属于放线菌门、拟杆菌门、厚壁菌门、γ变形菌门和盐古细菌门。从草底氧层提取的16S rRNA细菌序列属于拟杆菌门、绿杆菌门和变形细菌门，而古细菌序列属于绿古细菌门和盐古细菌门。来自同一垫的单一培养蓝藻与不同的AH相关，尽管在大多数培养中发现拟杆菌门。CARD-FISH结果显示拟杆菌门和绿柔相关细菌与微藻藻丝密切相关。通过添加从非无菌微结肠菌培养液中获得的滤液，并仅含有AH和释放物质，刺激了chthonoplastes PCC7420的无菌培养物的生长。相比之下，来自非无菌蓝藻相关培养物的类似滤液杀死了蓝藻PCC 7418。我们的结论是，在微生物席中存在着一个与蓝藻密切相关的多样化的酸碱菌群落，酸碱菌和蓝藻之间的相互作用是物种特异性的，涉及物质的释放。
Aerobic heterotrophic microorganisms (AH) play a significant role in carbon cycling in cyanobacterial mats; however, little is known about their abundance, diversity and interaction with cyanobacteria. Using catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), bacterial counts in the mat's oxic layer reached a mean of 2.23 +/- 0.4 x 10(10) cells g(-1). Cultivation of AH yielded strains belonging to Actinobacteria, Bacteroidetes, Firmicutes, Gammaproteobacteria and Haloarchaea. 16S rRNA bacterial sequences retrieved from the mat's oxic layer were related to Bacteroidetes, Chloroflexi and Proteobacteria, whereas archaeal sequences belonged to Crenarchaeota and Haloarchaea. Monocultures of cyanobacteria from the same mat were associated with different AH, although Bacteroidetes were found in most cultures. CARD-FISH showed that Bacteroidetes- and Chloroflexi-related bacteria were closely associated with filaments of Microcoleus chthonoplastes. The growth of an axenic culture of M. chthonoplastes PCC7420 was stimulated on the addition of a filtrate obtained from a non-axenic Microcoleus culture and containing only AH and released substances. In contrast, a similar filtrate from a non-axenic Cyanothece-related culture killed Cyanothece PCC 7418. We conclude that a diverse community of AH exist in close association with cyanobacteria in microbial mats and the interactions between AH and cyanobacteria are species-specific and involve the release of substances.