Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines.
Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines.
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DOI：
10.1002/0471142735.im0722s76
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发表时间：
2007-02-01
影响因子：
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通讯作者：
Cohen, Jeffrey I
中科院分区：
文献类型：
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作者：
Tosato, Giovanna;Cohen, Jeffrey I
EBV使B淋巴细胞永生化是诱导某些人B淋巴细胞长期生长的有效方法。本单元中描述的实现这一目的的方案可以分为三个阶段：病毒的制备，待永生化的靶细胞的制备，以及EBV感染和感染细胞的生长。
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.