GROWTH SUPPRESSION INDUCED BY WILD-TYPE P53-PROTEIN IS ACCOMPANIED BY SELECTIVE DOWN-REGULATION OF PROLIFERATING-CELL NUCLEAR ANTIGEN EXPRESSION
GROWTH SUPPRESSION INDUCED BY WILD-TYPE P53-PROTEIN IS ACCOMPANIED BY SELECTIVE DOWN-REGULATION OF PROLIFERATING-CELL NUCLEAR ANTIGEN EXPRESSION
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DOI：
10.1073/pnas.88.5.1958
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发表时间：
1991-03-01
影响因子：
11.1
通讯作者：
ULLRICH, SJ
中科院分区：
文献类型：
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作者：
MERCER, WE;SHIELDS, MT;ULLRICH, SJ
p53基因是多种人类癌症中常见的突变靶点。 以前，据报道，在来源于人多形性胶质母细胞瘤肿瘤的细胞系（GM47.23）中条件性表达野生型p53蛋白对细胞增殖具有负面影响。 我们现在已经研究了在该细胞系中诱导野生型p53蛋白对增殖细胞核抗原基因表达的影响。 增殖细胞核抗原基因编码一种核蛋白，它是DNA聚合酶-δ的辅助因子，也是细胞DNA复制机制的一部分。 我们发现，抑制细胞周期进入S期后，诱导野生型p53蛋白伴随着选择性下调增殖细胞核抗原mRNA和蛋白质的表达。
The p53 gene is a frequent target of mutation in a wide variety of human cancers. Previously, it was reported that conditional expression of wild-type p53 protein in a cell line (GM47.23) derived from a human glioblastoma multiform tumor had a negative effect on cell proliferation. We have now investigated the effect that induction of wild-type p53 protein in this cell line has on the expression of the proliferating-cell nuclear antigen gene. The proliferating-cell nuclear antigen gene encodes a nuclear protein that is an auxiliary factor of DNA polymerase-delta and part of the DNA replication machinery of the cell. We show that inhibition of cell cycle progression into S-phase after induction of wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen mRNA and protein expression.