Seminal Transmission of Lumpy Skin Disease Virus in Heifers
Seminal Transmission of Lumpy Skin Disease Virus in Heifers
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DOI：
10.1111/tbed.12045
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发表时间：
2014-10-01
影响因子：
4.3
通讯作者：
Venter, E. H.
中科院分区：
文献类型：
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作者：
Annandale, C. H.;Holm, D. E.;Venter, E. H.
众所周知，红斑狼疮病毒（LSDV）可在感染后在公牛精液中脱落，人工授精（AI）也会造成生物安全风险。然而，目前尚不清楚在人工智能中使用受LSDV感染的精液是否会构成生物安全风险。本研究的目的是调查是否LSDV，通过精液传播，可以感染奶牛及其胚胎。同时进行了两项对照试验。使用OvSynch方案对11头未感染LSDV的年轻小母牛进行同步化，并在第0天用加有LSDV野外菌株的新鲜精液进行授精。在第1天使用妊娠母马血清促性腺激素对6头小母牛进行超数排卵，并在第6天从这些小母牛中冲洗胚胎。从第4天至第27天收集血液和血清样品，以通过PCR和病毒分离确定LSDV的存在，并通过SNT确定抗LSDV抗体的存在。在第10天观察到LSD的首次临床体征，随后在3头小母牛中观察到重度全身LSD，在另外2头小母牛中观察到轻度LSD。由于严重的无反应性淋尿症，对两头小母牛实施人道安乐死。通过PCR、病毒分离或电子显微镜在实验感染动物的血液、胚胎和器官中检测到LSDV; 8头小母牛在第27天发生血清转化。两只对照动物未受影响。这是首次报道LSDV在牛中的实验性精液传播。
It is known that lumpy skin disease virus (LSDV) can be shed in bull semen following infection and also that artificial insemination (AI) poses a biosecurity risk. However, it is not known whether the use of LSDV infected semen in AI poses a biosecurity risk. The aim of this study was to investigate whether LSDV, transmitted through semen, can infect cows and their embryos. Two controlled trials were performed simultaneously. Eleven young beef heifers, naive to LSDV, were synchronized using an OvSynch protocol and inseminated on Day 0 with fresh semen spiked with a field strain of LSDV on day 0. Six of the heifers were superovulated on Day 1 using pregnant mare serum gonadotropin, and embryos were flushed from these heifers on Day 6. Blood and serum samples were collected from Day 4 until Day 27 to determine the presence of LSDV by PCR and virus isolation, and the presence of antibodies against LSDV by SNT. The first clinical signs of LSD were noticed on Day 10, followed by severe generalized LSD in three heifers and mild LSD in two more heifers. Two heifers were humanely euthanized due to severe unresponsive stranguria. LSDV was detected by PCR, virus isolation or electron microscopy in blood, embryos and organs of experimentally infected animals; and eight heifers had seroconverted by Day 27. Two control animals were not affected. This is the first report of experimental seminal transmission of LSDV in cattle.