Fabrication and characterisation of PCL and PCL/PLA scaffolds for tissue engineering
Fabrication and characterisation of PCL and PCL/PLA scaffolds for tissue engineering
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DOI：
10.1108/rpj-04-2012-0037
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发表时间：
2014-01-01
影响因子：
3.9
通讯作者：
Bartolo, P. J.
中科院分区：
文献类型：
--
作者：
Patricio, T.;Domingos, M.;Bartolo, P. J.
目的-本研究工作的主要目的是研究聚乳酸（PLA）添加到聚（ε-己内酯）（PCL）基质，以及混合过程对形态、热、化学，用新型生物制造设备生产的3D结构的机械和生物性能设计/方法/途径-使用两种混合工艺来制备PCL/PLA共混物，即熔融共混和溶剂浇铸。PCL和PCL/PLA支架通过BioCell Printing使用300 μ m喷嘴、0/90度铺设模式和350 μ m孔径生产。几种技术，如扫描电子显微镜（SEM），同步热分析仪（STA），核磁共振（NMR），静态压缩分析和Alcohol BlueTM被用来评估支架的形态，热，化学，机械和生物学性能。此外，通过溶剂浇铸获得的聚合物共混物呈现出更好的机械和生物性能，相比，通过熔融共混制备的共混物。独创性/价值-本文进行了详细的研究混合过程对PCL/PLA支架的生物力学性能的影响。结果将能够制备定制的PCL/PLA支架用于组织工程应用，与单独的PCL支架相比，具有改善的生物学和机械性能。此外，BioCell Printing的准确性和再现性能够调节支架的微观/宏观结构，增强组织再生。
Purpose - The main purpose of this research work is to study the effect of poly lactic acid (PLA) addition into poly (e-caprolactone) (PCL) matrices, as well the influence of the mixing process on the morphological, thermal, chemical, mechanical and biological performance of the 3D constructs produced with a novel biomanufacturing device (BioCell Printing).Design/methodology/approach - Two mixing processes are used to prepare PCL/PLA blends, namely melt blending and solvent casting. PCL and PCL/PLA scaffolds are produced via BioCell Printing using a 300-mu m nozzle, 0/90 degrees lay down pattern and 350-mu m pore size. Several techniques such as scanning electron microscopy (SEM), simultaneous thermal analyzer (STA), nuclear magnetic resonance (NMR), static compression analysis and Alamar BlueTM are used to evaluate scaffold's morphological, thermal, chemical, mechanical and biological properties.Findings - Results show that the addition of PLA to PCL scaffolds strongly improves the biomechanical performance of the constructs. Additionally, polymer blends obtained by solvent casting present better mechanical and biological properties, compared to blends prepared by melt blending.Originality/value - This paper undertakes a detailed study on the effect of the mixing process on the biomechanical properties of PCL/PLA scaffolds. Results will enable to prepare customized PCL/PLA scaffolds for tissue engineering applications with improved biological and mechanical properties, compared to PCL scaffolds alone. Additionally, the accuracy and reproducibility of by the BioCell Printing enables to modulate the micro/macro architecture of the scaffolds enhancing tissue regeneration.