QTL mapping of genes controlling ear emergence time and plant height on chromosome 5A of wheat
QTL mapping of genes controlling ear emergence time and plant height on chromosome 5A of wheat
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DOI：
10.1007/s001220051094
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发表时间：
1999-03-01
影响因子：
5.4
通讯作者：
Sawada, S
中科院分区：
文献类型：
--
作者：
Kato, K;Miura, H;Sawada, S
小麦的 5A 染色体携带农艺性状的主要基因位点，包括春化要求 Vrn-A1) 和穗形态 (Q)。为了确定穗出苗时间和株高的遗传变异是否归因于这些主要基因（作为多效性效应或独立QTL），我们将由源自“中国春”（Cappelle-Desprez 5A）和CS（Triticum spelta 5A）杂交的120个重组替代品系构建的RFLP图谱与从3年多的试验中收集的数据结合起来。对于穗出苗时间，对开花时间的主要影响是 Vrn-A1 和 QEet.ocs5A.1，后者是 28.6-cM Xcdo584/Q 间隔中的 QTL，与 Q 连锁小于 10 cM。 QEet.ocs-5A.1 上的 CS(T. spelta 5A) 等位基因使穗出现时间提前 2.7-6.0 天，这大约等于 Vrn-A1 的影响。对于株高，在长臂上鉴定出三个 QTL，并以排斥方式连锁。 Vrn-A1 处的 CS(T. spelta 5A) 等位基因或与 Xfba068 紧密连锁的 CS(T. spelta 5A) 等位基因导致高度降低 3.5-6.1 cm，而来自 CS(Cappelle-Desprez 5A) 的 Xcdo1088/Xbcd9 区间内的 Q 等位基因和 Qt.ocs-5A.1 等位基因产生的植株较短。当株高划分为秆长度和穗长度时，发现 Xcd1088/Xbcd9 区间内 QCl.ocs-5A.1 上的 Vrn-A1 等位基因和 CS(Cappelle-Desprez 5A) 等位基因导致秆较短。 q 处的 CS(T. spelta 5A) 等位基因是长耳朵的主要决定因素，同时 QEl.ocs-5A1 处的影响较小。在 Xcdo1088/Xbcd9 间隔内。
Chromosome 5A of wheat carries major gene loci for agronomic traits including the vernalization requirement Vrn-A1) and ear morphology (Q). To determine whether the genetic variation for ear emergence time and plant height is attributable to either of these major genes as pleiotropic effects or independent QTL, we combined a RFLP map constructed from 120 recombinant substitution lines derived from a cross between 'Chinese Spring' (Cappelle-Desprez 5A) and CS(Triticum spelta 5A) with data collected from held trials over 3 years. For ear emergence time the main effects on flowering time were by Vrn-A1 and QEet.ocs5A.1, the latter a QTL in the 28.6-cM Xcdo584/Q interval linked to Q by less than 10 cM. The CS(T. spelta 5A) allele at QEet.ocs-5A.1 contributed to an earlier ear emergence time by 2.7-6.0 days, which was approximately equal to the effects of Vrn-A1. For plant height, three QTLs were identified on the long ann and linked in repulsion. The CS(T. spelta 5A) allele at Vrn-A1 or closely linked to Xfba068 contributed to a height reduction of 3.5-6.1 cm, whereas both the Q allele and Qt.ocs-5A.1 allele within the Xcdo1088/Xbcd9 interval from CS(Cappelle-Desprez 5A) produced a shorter plant. When plant height was partitioned into culm length and ear length, the Vrn-A1 allele and CS(Cappelle-Desprez 5A) allele at QCl.ocs-5A.1 within the Xcd1088/Xbcd9 interval were found to contribute to a shorter culm. CS(T. spelta 5A) allele at q was a major determinant of a long ear, together with minor effects at QEl.ocs-5A1. within the Xcdo1088/Xbcd9 interval.