Limited ERG11 Mutations Identified in Isolates of Candida auris Directly Contribute to Reduced Azole Susceptibility.
Limited ERG11 Mutations Identified in Isolates of Candida auris Directly Contribute to Reduced Azole Susceptibility.
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DOI：
10.1128/aac.01427-18
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发表时间：
2018-10
影响因子：
4.9
通讯作者：
Perlin DS
中科院分区：
文献类型：
--
作者：
Healey KR;Kordalewska M;Jiménez Ortigosa C;Singh A;Berrío I;Chowdhary A;Perlin DS
在来自印度和哥伦比亚的耳念珠菌临床分离株中发现了多个Erg11氨基酸取代。在异源表达C.然而，编码I466 M、Y501 H或其他进化枝定义的氨基酸差异的等位基因的表达产生了易感MIC。在源自印度和哥伦比亚的耳念珠菌临床分离株中鉴定出多个Erg11氨基酸取代。在异源表达C.然而，编码I466 M、Y501 H或其他进化枝定义的氨基酸差异的等位基因的表达产生了易感MIC。耳ERG 11突变直接导致唑类药物敏感性降低。
Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. auris ERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Similar to other Candida species, specific C. auris ERG11 mutations resulted directly in reduced azole susceptibility.