Assessing candidate DNA barcodes for Chinese and internationally traded timber species
Assessing candidate DNA barcodes for Chinese and internationally traded timber species
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评估中国和国际贸易木材树种的候选 DNA 条形码
DOI：
10.1111/1755-0998.13546
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发表时间：
2021-11-23
影响因子：
7.7
通讯作者：
Li, Jie
中科院分区：
文献类型：
--
作者：
Hu, Jian-Lin;Ci, Xiu-Qin;Li, Jie
准确识别木材中的物种是帮助控制非法采伐和森林损失的重要步骤。然而，目前的木材识别方法的基础上形态和解剖特征有限的物种分辨率。DNA条形码是植物物种鉴定的一种行之有效的工具，但需要建立广泛的分类和空间尺度的可靠参考数据。在这里，我们构建了一个物种条形码库，包括1550个分类上不同的木材物种，来自656属和124科，代表了一个全面的遗传参考数据集，为中国木材物种和国际商业贸易木材物种，使用4个条形码（rbcL，matK，trnH-psbA，和ITS 2）。ITS 2片段被认为是最有效的位点中的中国木材种鉴定的四个条形码测试，无论是在种和属的水平，尽管其回收率低。尽管如此，条码组合matK+trnH-psbA+ ITS 2需要作为一个互补的条码，以区分密切相关的物种在复杂的数据集，涉及国际贸易的木材物种。科级鉴别和种/属比的比较分析表明，包含密切相关的物种是影响木材物种验证条形码分辨率的重要因素。我们的研究表明，虽然核ITS 2是最有效的单一条形码在中国的木材物种鉴定，互补的组合，如matK+trnH-psbA+ ITS 2需要提供更广泛的区分力。这些新生成的序列丰富了现有的公开数据库，特别是热带和亚热带万年青用材树，目前的木材物种条形码参考库可以作为林业监测，非法采伐起诉和生物多样性项目的重要遗传资源。
Accurate identification of species from timber is an essential step to help control illegal logging and forest loss. However, current approaches to timber identification based on morphological and anatomical characteristics have limited species resolution. DNA barcoding is a proven tool for plant species identification, but there is a need to build reliable reference data across broad taxonomic and spatial scales. Here, we construct a species barcoding library consisting of 1550 taxonomically diverse timber species from 656 genera and 124 families, representing a comprehensive genetic reference data set for Chinese timber species and international commercial traded timber species, using four barcodes (rbcL, matK, trnH–psbA, and ITS2). The ITS2 fragment was found to be the most efficient locus for Chinese timber species identification among the four barcodes tested, both at the species and genus level, despite its low recovery rate. Nevertheless, the barcode combination matK+trnH–psbA+ITS2 was required as a complementary barcode to distinguish closely related species in complex data sets involving internationally traded timber species. Comparative analyses of family‐level discrimination and species/genus ratios indicated that the inclusion of closely related species is an important factor affecting the resolution ability of barcodes for timber species verification. Our study indicates that although nuclear ITS2 is the most efficient single barcode for timber species authentication in China, complementary combinations like matK+trnH–psbA+ITS2 are required to provide broader discrimination power. These newly‐generated sequences enrich the existing publicly available databases, especially for tropical and subtropical evergreen timber trees and this current timber species barcode reference library can serve as an important genetic resource for forestry monitoring, illegal logging prosecution and biodiversity projects.