Injectable thixotropic hydrogel comprising regenerated silk fibroin and hydroxypropylcellulose
Injectable thixotropic hydrogel comprising regenerated silk fibroin and hydroxypropylcellulose
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包含再生丝素蛋白和羟丙基纤维素的可注射触变水凝胶
DOI：
10.1039/c2sm06984a
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发表时间：
2012-01-01
期刊：
影响因子：
3.4
通讯作者：
Shao, Zhengzhong
中科院分区：
文献类型：
--
作者：
Gong, Zuguang;Yang, Yuhong;Shao, Zhengzhong
以再生丝素（SF）和羟丙基纤维素（HPC）为原料，制备了一种新型触变性注射水凝胶。动态振荡流变学研究表明，共混物在37℃下1 h内成胶，通过调节共混物的混合比例可控制凝胶动力学和凝胶性能。通过共聚焦激光扫描显微镜（CLSM）的形态观察、结构表征以及拉曼光谱和定量c -13核磁共振光谱的分子迁移率研究，分别阐明了这种共混物的凝胶化机理。结果表明，共混物的相分离触发了SF从随机线圈到β -片的构象转变，从而通过β -片交联形成凝胶网络，并使分子链固定在分散相中。此外，研究表明，在注射过程中，混合水凝胶可以保护被包裹的细胞免受高剪切力的影响，这表明SF-HPC水凝胶是一种很有前途的细胞递送载体。这种具有触变流变特性的可注射水凝胶有望潜在地克服与可注射水凝胶原位形成相关的液体前体渗漏到邻近组织的问题。
In this paper, a novel thixotropic injectable hydrogel has been developed by blending regenerated silk fibroin (SF) and hydroxypropylcellulose (HPC). Dynamic oscillatory rheology showed that the blends gelled at 37 degrees C within 1 h, and the gelation kinetics and gel properties were controllable by tuning the mix ratio of the blends. The gelation mechanism of such blends was elucidated from morphological observations by confocal laser scanning microscopy (CLSM), structural characterization and a molecular mobility study via Raman spectroscopy and quantitative C-13-NMR spectroscopy, respectively. The results suggested that the phase separation of the blends triggered the conformational transition of SF from random coil to beta-sheet, and thus resulted in the gel network formation through the beta-sheet crosslinks and the immobilization of the molecular chain in the dispersed phase. Moreover, it was demonstrated that the blend hydrogel could protect encapsulated cells against high shear force during injection, suggesting that the SF-HPC hydrogel is a promising vehicle for cell delivery. This injectable hydrogel with thixotropic rheological properties was expected to potentially overcome the problem of leakage of liquid precursors to neighboring tissues associated with the in situ formation of injectable hydrogels.