An oxidative environment promotes growth of Mycobacterium abscessus.
An oxidative environment promotes growth of Mycobacterium abscessus.
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DOI：
10.1016/j.freeradbiomed.2010.08.026
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发表时间：
2010-12-01
影响因子：
7.4
通讯作者：
Crapo, James D.
中科院分区：
文献类型：
--
作者：
Oberley-Deegan, Rebecca E.;Rebits, Brittany W.;Weaver, Michael R.;Tollefson, Angela K.;Bai, Xiyuan;McGibney, Mischa;Ovrutsky, Alida R.;Chan, Edward D.;Crapo, James D.
关键词：
结核分枝杆菌（Mycobacterium speedessus，M.肺结核感染，特别是那些引起慢性肺部疾病的感染，在世界范围内变得越来越普遍。M.由于抗生素耐药性，尿道感染很难治疗。因此，迫切需要新的治疗方案。M.寄生虫是主要感染巨噬细胞和成纤维细胞的细胞内病原体。由于这种细菌最近才被确定为一个单独的物种，因此对M.寄生虫与宿主的相互作用以及M.葡萄的生长受到调控。长期以来，氧化应激被证明可以抑制细菌生物体的生长。然而，一些细胞内细菌，如结核分枝杆菌，在氧化环境中生长良好。在本研究中，我们表明，M。寄生虫感染导致宿主细胞环境变得更加氧化。此外，我们表明，一个更氧化的环境导致增强的增长M。巨噬细胞内的脂肪。在抗氧化剂MnTE-2-PyP（化学名：Meso-Tetrakis-（N-Methylpyridinium-2-yl）porphryin）或N-乙酰基-L-半胱氨酸（NAC）存在下，M.菌丝生长受到抑制。这些结果使我们假设抗氧化剂可能有助于治疗M。尿道感染。
Mycobacterium abscessus (M. abscessus) infections, particularly those causing chronic lung diseases, are becoming more prevalent worldwide. M. abscessus infections are difficult to treat due to antibiotic resistance. Thus, new treatment options are urgently needed. M. abscessus are intracellular pathogens that primarily infect macrophages and fibroblasts. Because this bacterium has only recently been identified as a separate species, very little is known about M. abscessus-host interactions and how M. abscessus growth is regulated. Oxidative stress has long been shown to inhibit growth of bacterial organisms. However, some intracellular bacteria, such as Mycobacterium tuberculosis, grow well in oxidizing environments. In the present study, we show that M. abscessus infection causes the host cell environment to become more oxidizing. Furthermore, we show that a more oxidizing environment leads to enhanced growth of M. abscessus inside macrophages. In the presence of the antioxidants, MnTE-2-PyP (chemical name: Manganese (II) Meso-Tetrakis-(N-Methylpyridinium-2-yl) porphryin) or N-acetyl-L-cysteine (NAC), M. abscessus growth is inhibited. These results lead us to postulate that antioxidants may aid in the treatment for M. abscessus infections.
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