Anxiety in adult female mice following perinatal exposure to chlorpyrifos
Anxiety in adult female mice following perinatal exposure to chlorpyrifos
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DOI：
10.1016/j.ntt.2009.08.008
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
Plumier, Jean-Christophe
中科院分区：
文献类型：
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作者：
Braquenier, Jean-Baptiste;Quertemont, Etienne;Plumier, Jean-Christophe
流行病学研究表明，产前暴露于有机磷杀虫剂（OP）和长期的精神延迟和一些行为问题之间可能存在联系。大鼠和小鼠的实验研究证实，在特定的围产期，相对较短的暴露于低剂量的OP，如毒死蜱（CPF），减少焦虑样行为。在本研究中，我们报告说，慢性围产期暴露（GD 15-PND 14）低剂量的CPF导致增加（而不是减少）的焦虑样行为的雌性小鼠仔鼠。怀孕或哺乳期的雌性小鼠暴露于CPF（0.2; 1;或5毫克/千克天），通过口服治疗，在连续18天。经过几周的恢复期后，使用神经行为测试（高架十字迷宫和明/暗盒测试）确定成年雌性后代的焦虑。我们的研究结果表明，暴露于CPF的雌性后代比对照组更焦虑。此外，本发明还提供了一种方法，焦虑特征改变的程度取决于妊娠期和哺乳期暴露于CPF的水平，在1 mg/kg/天剂量下观察到最大效应。我们的结果证实，围产期暴露于OP可诱导小鼠焦虑的长期改变。喜欢的行为，并建议在大脑发育过程中的管理途径和OP暴露的持续时间可能是考虑因素时，研究焦虑的发展。(C)2009 Elsevier Inc. All rights reserved.
Epidemiologic studies suggested a possible link between prenatal exposure to organophosphate insecticides (OP) and long-term mental delay and some behavioral troubles. Experimental studies in rats and mice have confirmed that a relatively short exposure to low doses of OP such as chlorpyrifos (CPF) during specific perinatal periods decreased anxiety-like behaviors. In the present study, we report that chronic perinatal exposure (GD15-PND14) to low doses of CPF leads to an increase (and not a decrease) in anxiety-like behaviors of female mouse offspring.Pregnant or lactating female mice were exposed to CPF (0.2; 1; or 5 mg/kg day) by oral treatment during 18 consecutive days. Following a recovery period of several weeks, the anxiety of adult female offspring was determined using neurobehavioral tests (elevated plus-maze and light/dark box tests). Our results showed that CPF-exposed female offspring were more anxious than controls. In addition, the magnitude of anxiety profile alterations depended on the level of exposure to CPF during gestation and lactation with a maximal effect observed at the 1 mg/kg day dose.Our results confirm that OP exposure during the perinatal period can induce long-term alterations in mouse anxiety-like behaviors and suggest that the routes of administration and the duration of OP exposure during brain development may be factors to consider when studying the development of anxiety. (C) 2009 Elsevier Inc. All rights reserved.