HUMAN RESPIRATORY-TRACT SECRETIONS - MUCOUS GLYCOPROTEINS OF NON-PURULENT TRACHEOBRONCHIAL SECRETIONS, AND SPUTUM OF PATIENTS WITH BRONCHITIS AND CYSTIC-FIBROSIS
HUMAN RESPIRATORY-TRACT SECRETIONS - MUCOUS GLYCOPROTEINS OF NON-PURULENT TRACHEOBRONCHIAL SECRETIONS, AND SPUTUM OF PATIENTS WITH BRONCHITIS AND CYSTIC-FIBROSIS
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DOI：
10.1016/0003-9861(76)90419-7
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发表时间：
1976-01-01
影响因子：
3.9
通讯作者：
POLONY, I
中科院分区：
文献类型：
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作者：
BOAT, TF;CHENG, PW;POLONY, I
通过琼脂糖凝胶过滤从非脓性气管支气管分泌物和脓性痰中分离粘液糖蛋白，脓性痰经还原、羧甲基化，在脓性分泌物的情况下，用DNA酶处理。溶解和纯化的糖蛋白在二乙基氨基乙基纤维素上分级分离成2种主要（I，II）和2种次要（Ia，III）血型活性组分。组分I和II具有类似的碳水化合物和氨基酸组成，这是人类血型物质的典型组成。这两个组件在几个方面确实不同。组分I含有1.4-2.6%的硫酸盐，不抑制流感病毒血凝，而组分II含有7.1-7.8%的硫酸盐，是病毒血凝的有效抑制剂。组分II在十二烷基硫酸钠-3.3%丙烯酰胺凝胶电泳上也迁移得更快。化脓性分泌物中的组分I和II与非化脓性分泌物中的组分相比仅表现出微小的成分差异。2例囊性纤维化患者的痰液样本中II组分含量高于化脓性支气管炎分泌物或非化脓性分泌物。
Mucous glycoproteins were isolated by agarose gel filtration from nonpurulent tracheobronchial secretions and purulent sputum which was reduced, carboxymethylated and, in the case of purulent secretions, treated with DNase. The solubilized and purified glycoproteins were fractionated on diethylaminoethyl cellulose into 2 major (I, II) and 2 minor (Ia, III) blood group active components. Components I and II had similar carbohydrate and amino acid compositions which were typical for human blood group substances. These 2 components did differ in several respects. Component I contained 1.4-2.6% sulfate and did not inhibit influenza virus hemagglutination while component II contained 7.1-7.8% sulfate and was a potent inhibitor of virus hemagglutination. Component II also migrated more rapidly on sodium dodecyl sulfate-3.3% acrylamide gel electrophoresis. Components I and II in purulent secretions displayed only minor compositional differences from their counterparts in nonpurulent secretions. Component II was more abundant in 2 sputum samples from subjects with cystic fibrosis than in purulent bronchitic secretions or in nonpurulent secretions.