Photosynthetic activity influences cellulose biosynthesis and phosphorylation of proteins involved therein in Arabidopsis leaves
Photosynthetic activity influences cellulose biosynthesis and phosphorylation of proteins involved therein in Arabidopsis leaves
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DOI：
10.1093/jxb/eru268
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发表时间：
2014-09-01
影响因子：
6.9
通讯作者：
Tcherkez, Guillaume
中科院分区：
文献类型：
--
作者：
Boex-Fontvieille, Edouard;Davanture, Marlene;Tcherkez, Guillaume
纤维素是陆地生态系统中最重要的有机化合物之一，是一种主要的植物结构聚合物。然而，对纤维素生物合成调控的认识仍然相当有限。最近的研究表明，纤维素酶（CESA）的磷酸化可能是纤维素生产中的关键调控事件。然而，环境条件对纤维素沉积的碳通量和CESA的磷酸化水平的影响尚未完全阐明。在这里，我们利用气体交换测量，同位素技术，代谢组学，和定量磷酸蛋白质组学研究拟南芥莲座叶中的纤维素生产的调节在不同的光合环境（不同的CO2摩尔分数）或光/暗转换。我们表明，碳通量纤维素生产增加光合作用，但不成比例。与CESA 1和3相关的几种磷酸肽和几种糖代谢酶的磷酸化水平在光照下较高和/或随着光合作用而增加。相比之下，与CESA 5相关的磷酸肽（Ser126）在黑暗中似乎更磷酸化。我们的数据表明，光合活性影响纤维素沉积通过控制蔗糖代谢和纤维素合成复合物本身的蛋白磷酸化。
Cellulose is one of the most important organic compounds in terrestrial ecosystems and represents a major plant structural polymer. However, knowledge of the regulation of cellulose biosynthesis is still rather limited. Recent studies have shown that the phosphorylation of cellulose synthases (CESAs) may represent a key regulatory event in cellulose production. However, the impact of environmental conditions on the carbon flux of cellulose deposition and on phosphorylation levels of CESAs has not been fully elucidated. Here, we took advantage of gas exchange measurements, isotopic techniques, metabolomics, and quantitative phosphoproteomics to investigate the regulation of cellulose production in Arabidopsis rosette leaves in different photosynthetic contexts (different CO2 mole fractions) or upon light/dark transition. We show that the carbon flux to cellulose production increased with photosynthesis, but not proportionally. The phosphorylation level of several phosphopeptides associated with CESA1 and 3, and several enzymes of sugar metabolism was higher in the light and/or increased with photosynthesis. By contrast, a phosphopeptide (Ser126) associated with CESA5 seemed to be more phosphorylated in the dark. Our data suggest that photosynthetic activity affects cellulose deposition through the control of both sucrose metabolism and cellulose synthesis complexes themselves by protein phosphorylation.