Surface modification of gold nanoparticles with neuron-targeted exosome for enhanced blood-brain barrier penetration
Surface modification of gold nanoparticles with neuron-targeted exosome for enhanced blood-brain barrier penetration
复制标题
DOI：
10.1038/s41598-019-44569-6
复制
发表时间：
2019-06-04
影响因子：
4.6
通讯作者：
Namdeel, Katawut
中科院分区：
文献类型：
--
作者：
Khongkow, Mattaka;Yata, Teerapong;Namdeel, Katawut
金纳米粒子（AuNPs）由于其在治疗、成像和表面修饰方面的多功能特性而被广泛用作治疗诊断应用的纳米材料。在这项研究中，外泌体衍生膜的独特功能与合成AuNP相结合，用于靶向递送至脑细胞。在这里，我们报告了通过使用机械方法或挤出来创建这些新型纳米材料的来自基因工程哺乳动物细胞的脑靶向外泌体对AuNPs进行表面修饰。在用脑靶向外泌体制造后，AuNP的独特靶向性质通过它们在层流条件下与脑细胞的结合以及它们增强的穿过血脑屏障的运输来证明。在进一步证明其靶向脑细胞的能力时，体内生物发光成像显示，靶向外泌体涂覆的AuNP在静脉注射后在小鼠脑中积累。在这项工作中展示的具有脑靶向外泌体的合成AuNP的表面修饰代表了提供有效脑靶向的高度新颖和有效的策略，并显示了使用修饰的AuNP穿透脑的未来前景。
Gold nanoparticles (AuNPs) have been extensively used as nanomaterials for theranostic applications due to their multifunctional characteristics in therapeutics, imaging, and surface modification. In this study, the unique functionalities of exosome-derived membranes were combined with synthetic AuNPs for targeted delivery to brain cells. Here, we report the surface modification of AuNPs with braintargeted exosomes derived from genetically engineered mammalian cells by using the mechanical method or extrusion to create these novel nanomaterials. The unique targeting properties of the AuNPs after fabrication with the brain-targeted exosomes was demonstrated by their binding to brain cells under laminar flow conditions as well as their enhanced transport across the blood brain barrier. In a further demonstration of their ability to target brain cells, in vivo bioluminescence imaging revealed that targeted-exosome coated AuNPs accumulated in the mouse brain after intravenous injection. The surface modification of synthetic AuNPs with the brain-targeted exosome demonstrated in this work represents a highly novel and effective strategy to provide efficient brain targeting and shows promise for the future in using modified AuNPs to penetrate the brain.