Expression of adipocytokines at sites of bone remodelling in rheumatoid arthritis
Expression of adipocytokines at sites of bone remodelling in rheumatoid arthritis
复制标题
类风湿性关节炎骨重建部位脂肪细胞因子的表达
DOI：
10.1136/annrheumdis-2011-201237.21
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发表时间：
2012
影响因子：
27.4
通讯作者：
Neumann E
中科院分区：
文献类型：
--
作者：
Krumbholz G;Lehr A;Rickert M;Rehart S;Schett G;Müller-Ladner U;Neumann E
研究背景类风湿性关节炎（RA）与脂肪细胞因子的产生增加有关，脂肪细胞因子是类胡萝卜素的介质。类风湿关节炎患者滑液中脂肪因子脂联素和脂联素水平的增加以及它们对骨重塑细胞（例如，成骨细胞、破骨细胞）的激活/抑制作用提出了这些细胞因子对骨吸收的免疫调节特性的问题。在这项研究中，脂联素和脂联素的表达在关节腔室和细胞的骨重建在RA的网站进行了analysed.MethodsBone组织中获得RA患者在关节置换手术。制备脱钙和脱蜡骨组织的连续切片。免疫组织化学被用来确定在关节骨的骨重建部位的脂肪因子的定位，并用于识别表达脂肪因子脂联素和脂联素的细胞。此外，通过使用抗人ALP（碱性磷酸酶）抗体进行免疫组织化学染色以鉴定成骨细胞，并使用TRAP染色以鉴定破骨细胞。对于组织学的概述，Masson三色染色performed.ResultsAdiponectin和Bronchin检测在所有关节的RA患者的骨重建的网站。成骨细胞和破骨细胞表达脂联素和脂联素。这两种脂肪因子与成骨细胞共同定位在新形成的非矿化骨组织的边缘。有趣的是，可以观察到的成骨细胞中的ALP的表达的表达强度的相关性，这表明活跃的成骨细胞产生增加的量的BLORN.ConclusionsThe结果表明，BLORN和脂联素表达的成骨细胞和破骨细胞在骨侵蚀的网站。因此，这些细胞类型能够对骨组织中的BMPN和脂联素产生反应，这可能进一步促进这些细胞类型的分化和活化。鸣谢由德国研究学会资助（SPP 1468，IMMUNOBONE，NE 1174/6-1）。
BackgroundRheumatoid arthritis (RA) is associated with increased production of adipocytokines, which are cytokine-like mediators. Increased levels of the adipokines adiponectin and resistin in synovial fluids of RA patients as well as their activating/inhibitory effect on cells of bone remodeling (eg, osteoblasts, osteoclasts) raise the question of immunomodulatory properties of these cytokines towards bone resorption. In this study, the expression of adiponectin and resistin in joint compartments and cells at the sites of bone remodelling in RA was analysed.MethodsBone tissue was obtained from RA patients during joint replacement surgery. Serial sections of decalcified and deparaffinised bone tissue were prepared. Immunohistochemistry was used to identify the localisation of adipokines at sites of bone remodelling in articular bone and for identification of cells expressing the adipokines adiponectin and resistin. Additionally, immunohistochemical staining was carried out by using antihuman ALP (alkaline phosphatase) antibodies for identifying osteoblasts and TRAP staining to identify osteoclasts. For histological overview, a Masson Trichrome staining was performed.ResultsAdiponectin and resistin were detectable in all joints of RA patients at sites of bone remodelling. An expression of adiponectin and resistin by osteoblasts and osteoclasts could be observed. Both adipokines were co-localised with osteoblasts at the border of newly formed, non-mineralised bone tissue. Interestingly, a correlation of the expression intensity of resistin with the expression of ALP in osteoblasts could be observed, indicating that active osteoblasts produce increased amounts of resistin.ConclusionsThe results indicate that resistin and adiponectin are expressed by osteoblasts and osteoclasts at sites of bone erosion. Therefore, these cell types are able to respond to resistin and adiponectin in bone tissue, which potentially further contribute to the differentiation and activation of these cell types.AcknowledgementFunded by the German Research Society (SPP1468, IMMUNOBONE, NE1174/6-1).