POLYMANNOSE O-ANTIGENS OF ESCHERICHIA-COLI, THE BINDING-SITES FOR THE REVERSIBLE ADSORPTION OF BACTERIOPHAGE-T5+ VIA THE L-SHAPED TAIL FIBERS
POLYMANNOSE O-ANTIGENS OF ESCHERICHIA-COLI, THE BINDING-SITES FOR THE REVERSIBLE ADSORPTION OF BACTERIOPHAGE-T5+ VIA THE L-SHAPED TAIL FIBERS
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DOI：
10.1128/jvi.41.1.222-227.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
BRAUN, V
中科院分区：
文献类型：
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作者：
HELLER, K;BRAUN, V
T5+ 和尾部无纤维突变体 hd-2 对各种大肠杆菌菌株脂多糖的吸附动力学研究表明，T5+ 与 O8 和 O9 类型的 O 抗原结合。将放射性甘露糖掺入磷酸甘露糖异构酶缺陷菌株大肠杆菌 F860 O9 pmi 中，可以推导出增加 T5 吸附所需的 O 抗原/细胞的数量。当 O 抗原分子超过 500 个时，观察到 T5+ 吸附加速。当几乎所有脂多糖分子都被多甘露糖O-抗原取代时，获得最高的吸附率。对 O8 型酶降解脂多糖的纯化成分进行的抑制研究表明，在测试的甘露糖苷中，最小单位三甘露糖苷是 T5+ 结合的最强抑制剂。因此，对 O8 和 O9 多聚甘露糖抗原的可逆预吸附增加了通过 fhuA（以前的 tonA）基因编码的细胞受体蛋白的感染率。
A study of the adsorption kinetics of T5+ and the tail fiber-less mutant hd-2 to lipopolysaccharides of various E. coli strains demonstrated T5+ binding to the O-antigen of the O8 and O9 types. Incorporation of radioactive mannose into the phosphomannose isomerase-deficient strain E. coli F860 O9 pmi allowed the derivation of the number of O-antigens/cell required to increase T5 adsorption. With > 500 O-antigen molecules, acceleration of T5+ adsorption was observed. The highest adsorption rate was obtained when nearly all lipopolysaccharide molecules were substituted with a polymannose O-antigen. Inhibition studies with purified components of an enzymatically degraded lipopolysaccharide of the O8 type showed that among the mannosides tested the smallest unit, the trimannoside, was the strongest inhibitor of T5+ binding. Thus, the reversible preadsorption to the O8 and O9 polymannose antigens increases the rate of infection via the cellular receptor protein encoded by the fhuA (formerly tonA) gene.