Wheat hypersensitive-induced reaction genes TaHIR1 and TaHIR3 are involved in response to stripe rust fungus infection and abiotic stresses
Wheat hypersensitive-induced reaction genes TaHIR1 and TaHIR3 are involved in response to stripe rust fungus infection and abiotic stresses
复制标题
小麦过敏诱导反应基因TaHIR1和TaHIR3参与对条锈菌感染和非生物胁迫的反应
DOI：
10.1007/s00299-012-1361-6
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发表时间：
2013-02-01
影响因子：
6.2
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
--
作者：
Duan, Yinghui;Guo, Jun;Kang, Zhensheng
关键信息：TaHIR1 和 TaHIR3 通过诱导 HR 和调节防御相关基因在抵抗条锈菌方面发挥积极作用，但受到各种非生物刺激的负向调节。已知植物过敏诱导反应（HIR）基因与过敏反应和疾病防御相关。在小麦中，两个 HIR 基因 TaHIR1 和 TaHIR3 已被鉴定，并发现在感染条锈菌后表达上调。在这里，我们进一步确定了它们在防御非生物胁迫和条锈病病原体 Puccinia striiformis f 中的作用。 sp。小麦。 TaHIR1 和 TaHIR3 蛋白定位于烟草细胞的质膜中。 TaHIR1和TaHIR3的表达因环境刺激（包括低温、干旱和高盐胁迫）而降低。此外，外源应用乙烯利和脱落酸下调TaHIR1和TaHIR3的表达，而水杨酸和茉莉酸甲酯处理不影响表达。此外，大麦条纹花叶病毒诱导的 TaHIR1 和 TaHIR3 基因沉默降低了小麦品种 Suwon11 对无毒条纹锈病 CYR23 和感染部位附近坏死细胞区域的抗性，并改变了防御相关基因的表达水平。这些结果表明，TaHIR1 和 TaHIR3 在小麦条锈菌的不相容相互作用中发挥积极作用，但对非生物胁迫表现出负转录反应。
KEY MESSAGE : TaHIR1 and TaHIR3 play positive roles in resistance to the stripe rust fungus via inducing HR and regulating defense-related genes, but are negatively regulated by various abiotic stimuli. Plant hypersensitive-induced reaction (HIR) genes are known to be associated with the hypersensitive response and disease defense. In wheat, two HIR genes, TaHIR1 and TaHIR3, have been identified and found to be up-regulated after infection with the stripe rust fungus. Here, we further determined their roles in defense against abiotic stresses and the stripe rust pathogen, Puccinia striiformis f. sp. tritici. TaHIR1 and TaHIR3 proteins were localized in the plasma membrane of tobacco cells. The expression of TaHIR1 and TaHIR3 was reduced by the environmental stimuli, including low temperature, drought, and high salinity stresses. In addition, the expression of TaHIR1 and TaHIR3 was down-regulated by exogenously applied ethrel and abscisic acid, whereas expression was not affected by treatments with salicylic acid and methyl jasmonate. Furthermore, barley stripe mosaic virus-induced gene silencing of TaHIR1 and TaHIR3 reduced resistance in wheat cultivar Suwon11 against an avirulent stripe rust pathotype CYR23 and area of necrotic cells neighboring the infection sites, and altered the expression levels of defense-related genes. These results suggest that TaHIR1 and TaHIR3 function positively in the incompatible interaction of wheat-stripe rust fungus, but exhibit negative transcriptional response to abiotic stresses.