Regulation of acid-base transporters by vasopressin in the kidney collecting duct of Brattleboro rat.
Regulation of acid-base transporters by vasopressin in the kidney collecting duct of Brattleboro rat.
复制标题
布拉特尔伯勒大鼠肾集合管中加压素对酸碱转运蛋白的调节。
DOI：
10.1159/000093305
复制
发表时间：
2006
影响因子：
4.2
通讯作者：
Soleimani,Manoocher
中科院分区：
文献类型：
--
作者：
Amlal,Hassane;Sheriff,Sulaiman;Faroqui,Somia;Ma,Liyun;Barone,Sharone;Petrovic,Snezana;Soleimani,Manoocher
目的研究长期加压素治疗对大鼠肾脏集合管酸碱转运蛋白的影响。方法将Brattleboro大鼠置于代谢笼中，每日注射选择性V2受体激动剂1-脱氨基-8-D-精氨酸加压素（dDAVP），结果dDAVP处理后血清碳酸氢根浓度显著降低，尿NH 4+排泄量增加，同时引起关键产氨酶表达上调，尿NH 4+排泄量沿着增加。北方杂交和免疫荧光标记显示，在dDAVP处理的大鼠中，顶部Cl-/HCO 3-交换器pendrin（PDS）的mRNA表达显著增加（+ 80%），沿着其蛋白丰度在皮质集合管中的B型闰细胞中急剧增加。dDAVP处理组大鼠延髓内集合管基底外侧Cl-/HCO 3-交换器（AE 1）和顶端H+-ATP酶的丰度显著降低。肾脏肾素mRNA显著增加，并与dDAVP注射大鼠血清醛固酮水平的增加相关。然而，血清皮质酮水平降低，并与肾脏11β-羟基类固醇脱氢酶-2 mRNA水平升高相关结论dDAVP慢性给药可使大鼠肾上腺PDS表达上调，集合管H+-ATP酶和AE 1表达下调。沿着氨生成的增加。肾素-血管紧张素-醛固酮系统的刺激和/或糖皮质激素水平的降低可能在这些效应的转导中起作用。
AimThe objective of these studies was to examine the effects of long-term vasopressin treatment on acid-base transporters in the collecting duct of rat kidney.MethodsBrattleboro rats were placed in metabolic cages and treated with daily injections of 1-desamino-8-D-arginine vasopressin (dDAVP), a selective V 2-receptor agonist, or its vehicle (control) for up to 8 days.ResultsdDAVP treatment resulted in a significant reduction in serum bicarbonate concentration, and caused the upregulation of key ammoniagenesis enzymes, along with increased urinary NH 4+ excretion. Northern hybridization and immunofluorescence labeling indicated a significant increase (+ 80%) in mRNA expression of the apical Cl–/HCO 3–exchanger pendrin (PDS), along with a sharp increase in its protein abundance in B-type intercalated cells in the cortical collecting duct in dDAVP-treated rats. In the inner medullary collecting duct, the abundance of basolateral Cl–/HCO 3–exchanger (AE1) and apical H+-ATPase was significantly reduced in dDAVP-treated rats. Kidney renin mRNA increased significantly and correlated with an increase in serum aldosterone levels in dDAVP-injected rats. Serum corticosterone levels were, however, reduced and correlated with increased mRNA levels of renal 11β-hydroxysteroid dehydrogenase-2 (11β-HSD2) and decreased mRNA expression of 11β-hydroxylase in the adrenal gland of dDAVP-injected rats.ConclusionChronic administration of dDAVP to Brattleboro rats is associated with the upregulation of PDS and downregulation of H+-ATPase and AE1 in the collecting duct, along with increased ammoniagenesis. Stimulation of the renin-angiotensin-aldosterone system and/or decreased glucocorticoid levels likely plays a role in the transduction of these effects.