Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats
Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats
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DOI：
10.3168/jds.2011-4831
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发表时间：
2012-04-01
影响因子：
3.5
通讯作者：
Yanez-Ruiz, D. R.
中科院分区：
文献类型：
--
作者：
Abecia, L.;Toral, P. G.;Yanez-Ruiz, D. R.
已经测试了几种技术来减少肠道产甲烷，但很少有成功地用于牲畜的实际条件。此外，瘤胃甲烷产量减少对动物生产性能和牛奶质量的影响知之甚少。本研究旨在探讨饲喂溴氯甲烷（BROCHROCHROME，BROCHROCHROME）对奶山羊瘤胃甲烷产生、发酵模式、主要微生物类群丰度以及动物生产性能和乳成分的影响。将18只山羊分配到2个实验组，每组9只动物：每天以0.30 g/100 kg体重的剂量给药（给药+）或不给药（给药-）。从分娩开始至断奶后2周（10周），每天口服2次，每次剂量相等。断奶后，在聚碳酸酯室中连续2天（处理第57天和第58天）记录甲烷排放量。在第59天，收集单个瘤胃液样品用于挥发性脂肪酸（VFA）分析和通过实时PCR定量细菌、原生动物和古细菌数量。在第69天和第70天，记录每日产奶量，并收集样品，通过红外分光光度法测定脂肪、蛋白质、乳糖、酪蛋白和总固体浓度，通过气相色谱法测定脂肪酸组成。与未处理的动物相比，用DIM处理使甲烷产量降低了33%（21.6与14.4 L/kg DIM），尽管它不影响瘤胃细菌、原生动物和总产甲烷古菌的丰度。所观察到的消化过程的效率的改善是伴随着牛奶产量增加36%，可能是由于更多的丙酸型瘤胃发酵和VFA生产的增加。产奶量的增加并不伴随着脂肪、蛋白质或乳糖的浓度或产量的任何变化。尽管甲烷产量大幅下降，牛奶脂肪酸谱只有轻微的变化，表明瘤胃生物氢化途径没有受到影响。影响甲烷产生的终端生物化学途径的化合物值得进一步开发，以供将来在奶山羊领域的应用。
Several technologies have been tested to reduce enteric methanogenesis, but very few have been successfully used in practical conditions for livestock. Furthermore, the consequences of reduced rumen methane production on animal performance and milk quality are poorly understood. The aim of this work was to investigate the effect of feeding bromochloromethane (BCM), a halogenated aliphatic hydrocarbon with potential antimethanogenic activity, to dairy goats on rumen methane production, fermentation pattern, the abundance of major microbial groups, and on animal performance and milk composition. Eighteen goats were allocated to 2 experimental groups of 9 animals each: treated (BCM+) or not (BCM-) with 0.30 g of BCM/100 kg of body weight per day. The BCM was administered per os in 2 equal doses per day from parturition to 2 wk postweaning (10 wk). After weaning, methane emissions were recorded over 2 consecutive clays (d 57 and 58 on treatment) in polycarbonate chambers. On d 59, individual rumen fluid samples were collected for volatile fatty acid (VFA) analysis and quantification of bacterial; protozoal, and archaeal numbers by realtime PCR. On d 69 and 70, daily milk production was recorded and samples were collected for determination of fat, protein, lactose, casein, and total solids concentration by infrared spectrophotometry, and fatty acid composition by gas chromatography. Treatment with BCM reduced methane production by 33% (21.6 vs. 14.4 L/kg of DIM) compared with nontreated animals, although it did not affect the abundance of rumen bacteria, protozoa, and total methanogenic archaea. The observed improvement in the efficiency of digestive processes was accompanied by a 36% increase in milk yield, probably due to the more propionic type of rumen fermentation and an increase in VFA production. The increase in milk yield was not accompanied by any changes in the concentrations or yields of fat, protein, or lactose. Despite the substantial decrease in methane production, only minor changes in milk fatty acid profile were observed, suggesting that ruminal biohydrogenation pathways were not affected. Compounds that influence terminal biochemical pathways for methane production deserve further development for future application in the dairy goat sector.