Abnormalities of basement membrane on blood vessels and endothelial sprouts in tumors
Abnormalities of basement membrane on blood vessels and endothelial sprouts in tumors
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DOI：
10.1016/s0002-9440(10)63540-7
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发表时间：
2003-11-01
影响因子：
6
通讯作者：
McDonald, DM
中科院分区：
文献类型：
--
作者：
Baluk, P;Morikawa, S;McDonald, DM
肿瘤血管基底膜通常被描述为不完整或缺失，作为血管生成和抗血管生成分子的来源、生长因子结合的部位、血管生成的参与者和癌症治疗的潜在靶点，引起了人们的重新关注。本研究评估了三种小鼠肿瘤模型：自发性RIP-Tag2胰岛肿瘤、MCa-IV乳腺癌和Lewis肺癌血管基底膜的组成、范围和结构完整性。肿瘤血管采用内皮细胞标志物CD31、内皮素（CD105）、血管内皮生长因子受体-2和整合素α 5 （CD49e）的免疫组化染色进行鉴定。共聚焦显微镜研究显示，IV型胶原免疫反应性鉴定的基底膜覆盖了三种肿瘤血管表面的99.9%，与正常胰岛相同。层粘连蛋白、肠动蛋白/氮化原和纤维连接蛋白免疫反应性；同样普遍存在于肿瘤血管中。通过分析1 μ m共聚焦光学切片，发现基膜上的孔为
Often described as incomplete or absent, the basement membrane of blood vessels in tumors has attracted renewed attention as a source of angiogenic and anti-angiogenic molecules, site of growth factor binding, participant in angiogenesis, and potential target in cancer therapy. This study evaluated the composition, extent, and structural integrity of the basement membrane on blood vessels in three mouse tumor models: spontaneous RIP-Tag2 pancreatic islet tumors, MCa-IV mammary carcinomas, and Lewis lung carcinomas. Tumor vessels were identified by immunohistochemical staining for the endothelial cell markers CD31, endoglin (CD105), vascular endothelial growth factor receptor-2, and integrin alpha5 (CD49e). Confocal microscopic studies revealed that basement membrane identified by type IV collagen immunoreactivity covered >99.9% of the surface of blood vessels in the three tumors, just as in normal pancreatic islets. Laminin, entactin/nidogen, and fibronectin immunoreactivities; were similarly ubiquitous on tumor vessels. Holes in the basement membrane, found by analyzing 1-mum confocal optical sections, were