Fourier transform capillary electrophoresis with Laminar-Flow gated pressure injection
Fourier transform capillary electrophoresis with Laminar-Flow gated pressure injection
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DOI：
10.1021/ac0710026
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发表时间：
2007-09-01
影响因子：
7.4
通讯作者：
Chiu, Daniel T.
中科院分区：
文献类型：
--
作者：
Allen, Peter B.;Doepker, Byron R.;Chiu, Daniel T.
傅里叶变换毛细管电泳（FTCE）是一种提高毛细管电泳分离的信噪比和分辨率的方法。在FTCE中，在同一通道系统中进行多个同时CE分离，并使用单点检测器进行询问。为了通过实验说明FTCE在S/N比和分辨率方面提供的改进，我们进行了适度数量（5次）的多次进样和分离。我们表明，即使用这种少量的分离，S/N增加了2.9倍，理论塔板高度提高了30倍以上。我们证明了这种技术与激光诱导荧光检测，但各种各样的检测方法是兼容的FTCE。
Fourier transform capillary electrophoresis (FTCE) was developed as a method to improve signal-to-noise ratio (S/N) and resolution in capillary electrophoresis (CE) separation. In FTCE, multiple simultaneous CE separations were performed in the same channel system and interrogated using a single-point detector. To illustrate experimentally the improvement offered by FTCE in S/N ratio and resolution, we carried out a modest number (five) of multiple injections and separations. We show even with this small number of separations, S/N increased by a factor of 2.9, and theoretical plate height improved by a factor of more than 30. We demonstrated this technique with laser-induced fluorescence detection, but a wide variety of detection methods are compatible with FTCE.