RFLP MAPPING OF THE VERNALIZATION (VRN1) AND FROST-RESISTANCE (FR1) GENES ON CHROMOSOME 5A OF WHEAT
RFLP MAPPING OF THE VERNALIZATION (VRN1) AND FROST-RESISTANCE (FR1) GENES ON CHROMOSOME 5A OF WHEAT
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DOI：
10.1007/bf00222940
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发表时间：
1995-06-01
影响因子：
5.4
通讯作者：
SNAPE, JW
中科院分区：
文献类型：
--
作者：
GALIBA, G;QUARRIE, SA;SNAPE, JW
以霜敏、春化不敏感的代换系'中国春'为材料，通过杂交获得了一个单染色体重组系群体（Triticum spelta 5A）和一个抗冻、春化敏感的品系'中国春'（“Cheyenne”5A），并分别用于定位控制春化要求和抗冻性的基因Vrn 1和Fr1，相对于位于该染色体上的RFLP标记。Vrn 1和Fr1基因座位于5AL长臂的远端，与以往的观察相反，它们之间发生了重组，3个RFLP标记Xpsr426、Xcdo504和Xwg644与两者紧密连锁。Vrn1基因的定位表明，它与大麦第7（5H）染色体上的Sh2基因和黑麦第5R染色体上的Sp1基因具有同源性。
A population of single chromosome recombinant lines was developed from the cross between a frost-sensitive, vernalization-insensitive substitution line, 'Chinese Spring' (Triticum spelta 5A) and a frost-tolerant, vernalization-sensitive line, 'Chinese Spring' ('Cheyenne' 5A), and used to map the genes Vrn1 and Fr1 controlling vernalization requirement and frost tolerance, respectively, relative to RFLP markers located on this chromosome. The Vrn1 and Fr1 loci were located closely linked on the distal portion of the long arm of 5AL, but contrary to previous observations, recombination between them was found. Three RFLP markers, Xpsr426, Xcdo504 and Xwg644 were tightly linked to both. The location of Vrn1 suggests that it is homoeologous to other spring habit genes in related species, particularly the Sh2 locus on chromosome 7 (5H) of barley and the Sp1 locus on chromosome 5R of rye.