Simple on-line sample preconcentration technique for peptides based on dynamic pH junction in capillary electrophoresis-mass spectrometry
Simple on-line sample preconcentration technique for peptides based on dynamic pH junction in capillary electrophoresis-mass spectrometry
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DOI：
10.1016/j.chroma.2007.03.014
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发表时间：
2007-05-04
影响因子：
4.1
通讯作者：
Terabe, Shigeru
中科院分区：
文献类型：
--
作者：
Imami, Koshi;Monton, Maria Rowena N.;Terabe, Shigeru
本文报道了一种基于动态pH连接的毛细管电泳-质谱（CE-MS）在线样品预富集技术。对于肽分析，将样品溶解在pH值高于背景溶液（BGS）的溶液中，并作为长塞进样到毛细管中。样品基质和BGS之间的pH差异导致电泳过程中分析物迁移率的变化，导致其边界处的条带变窄。在峰强度方面，可以实现约550-1000倍的灵敏度增强，而不降低峰形和分辨率。该技术易于操作，将用于蛋白质分析中的肽质量指纹图谱。(C)2007 Elsevier B.V保留所有权利。
We report an on-line sample preconcentration technique based on dynamic pH junction in capillary electrophoresis-mass spectrometry (CE-MS). For peptide analysis, the samples were dissolved in a solution with higher pH than the background solution (BGS), and were injected into the capillary as a long plug. The pH difference between the sample matrix and BGS caused changes in analytes' mobilities during electrophoresis, resulting in narrowing of their bands at the boundary. Around 550-1000-fold sensitivity enhancement could be achieved in terms of peak intensity without degrading peak shape and resolution. This technique is easy to perform and will be useful for peptide mass fingerprinting in protein analysis. (C) 2007 Elsevier B.V All rights reserved.