A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency.
A hybrid substratum for primary hepatocyte culture that enhances hepatic functionality with low serum dependency.
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DOI：
10.2147/ijn.s75011
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发表时间：
2015
影响因子：
8
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Meng Q;Tao C;Qiu Z;Akaike T;Cui F;Wang X
细胞培养系统已被证明对于原代肝细胞的体外维持和高水平肝功能表达的维持至关重要。由于特定的半乳糖-脱唾液酸糖蛋白受体相互作用，聚（N-对乙烯基苄基-4-O-β-D-吡喃半乳糖基-D-葡萄糖酰胺）基质可以识别细胞并促进球状结构中的肝功能。同时，融合蛋白 E-cadherin-Fc 在与各种细胞一起孵育时，显示出对细胞活力和新陈代谢的增强作用。因此，通过使用这两种材料结合其原代肝细胞培养的优点，开发了一种用于生物医学应用的混合培养基。分离的细胞在共固定化表面上表现出单层聚集体形态，并且比传统基质上的细胞表现出更高的功能表达。此外，混合系统通过添加 1% 胎牛血清实现了最高水平的细胞粘附和肝细胞代谢，显示出比胶原/明胶涂层表面更低的血清依赖性。因此，该底物可以减弱血清的负面影响，并进一步有助于建立原代肝细胞的确定的培养系统。
Cell culture systems have proven to be crucial for the in vitro maintenance of primary hepatocytes and the preservation of hepatic functional expression at a high level. A poly-(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) matrix can recognize cells and promote liver function in a spheroid structure because of a specific galactose–asialoglycoprotein receptor interaction. Meanwhile, a fusion protein, E-cadherin-Fc, when incubated with various cells, has shown an enhancing effect on cellular viability and metabolism. Therefore, a hybrid substratum was developed for biomedical applications by using both of these materials to combine their advantages for primary hepatocyte cultures. The isolated cells showed a monolayer aggregate morphology on the coimmobilized surface and displayed higher functional expression than cells on traditional matrices. Furthermore, the hybrid system, in which the highest levels of cell adhesion and hepatocellular metabolism were achieved with the addition of 1% fetal bovine serum, showed a lower serum dependency than the collagen/gelatin-coated surface. Accordingly, this substrate may attenuate the negative effects of serum and further contribute to establishing a defined culture system for primary hepatocytes.