Selenium-induced changes in activities of antioxidant enzymes and content of photosynthetic pigments in Spirulina platensis
Selenium-induced changes in activities of antioxidant enzymes and content of photosynthetic pigments in Spirulina platensis
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硒诱导钝顶螺旋藻抗氧化酶活性和光合色素含量的变化
DOI：
10.1111/j.1744-7909.2007.00600.x
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发表时间：
2008-01-01
影响因子：
11.4
通讯作者：
Yang, Fang
中科院分区：
文献类型：
--
作者：
Chen, Tian-Feng;Zheng, Wen-Jie;Yang, Fang
不同硒浓度（0、10、20、40、80、150、175、200、250 mg/L）的钝顶螺旋藻（Spirulinaplatensis）对硒的积累具有剂量和时间依赖性。在低硒浓度（175 mg/L）条件下，硒的积累量增加，GPX、SOD、CAT和POD活性升高，但同时也导致膜脂过氧化（LPO）和钾渗漏，生物量和光合色素含量降低。结果表明，抗氧化酶活性的增加不足以保护细胞膜免受硒胁迫。在40和200 mg/L的硒浓度下，研究了抗氧化酶活性、叶绿素a和类胡萝卜素含量以及过氧化脂质水平随时间的变化。SOD-CAT活性和GPX-POD-APX活性在各生育期内呈相反的变化趋势。结果表明，防止S.通过SOD-CAT和GPX-POD-APX酶的协同作用，可以实现对钝顶螺旋藻细胞的诱导。本研究认为S.钝顶螺旋藻对硒具有耐受性，可以通过改变各种代谢过程来保护自己免受硒诱导的植物毒性。
Spirulina platensis exposed to various selenium (Se) concentrations (0, 10, 20, 40, 80, 150, 175, 200, 250 mg/L) accumulated high amounts of Se in a dose- and time-dependent manner. Under low Se concentrations (= 175 mg/L) led to higher Se accumulation and increases in activities of GPX, SOD, CAT and POD, but also induced lipid peroxidation (LPO) coupled with potassium leakage and decreases in biomass concentration and contents of photosynthetic pigment. The results indicate that increases in activities of the antioxidant enzymes were not sufficient to protect cell membranes against Se stress. Time-dependent variations in the activities of antioxidant enzymes, contents of chlorophyll a and carotenoid and the LPO level were also investigated under representative Se concentrations of 40 and 200 mg/L. Opposite variation trends between SOD-CAT activities, and GPX-POD-APX activities were observed during the growth cycles. The results showed that the prevention of damage to cell membranes of S. platensis cells could be achieved by cooperative effects of SOD-CAT and GPX-POD-APX enzymes. This study concludes that S. platensis possessed tolerance to Se and could protect itself from phytotoxicity induced by Se by altering various metabolic processes.