Quantification of the nonlinear susceptibility of the hydrogen and deuterium stretch vibration for biomolecules in coherent Raman micro-spectroscopy.
Quantification of the nonlinear susceptibility of the hydrogen and deuterium stretch vibration for biomolecules in coherent Raman micro-spectroscopy.
复制标题
定量氢和氘拉伸振动对相干拉曼微光谱法中生物分子的敏感性。
DOI：
10.1002/jrs.6164
复制
发表时间：
2021-09
影响因子：
2.5
通讯作者：
Langbein, Wolfgang
中科院分区：
文献类型：
--
作者：
Boorman, Dale;Pope, Iestyn;Masia, Francesco;Watson, Peter;Borri, Paola;Langbein, Wolfgang
为了提高化学特异度，在生物细胞和组织等复杂系统的相干拉曼成像中越来越多地使用氚标记。然而，以前还没有关于氚化合物的定量相干拉曼感度谱的报道。有趣的是，理论上预计-D伸缩振动的拉曼极化率低于-H伸缩振动，其虚部的面积随着波数的变化而变化，C-H的虚部面积从2900 cm−1左右移动到C-D的2100 cm−1附近的无声区。本文报道了水、琥珀酸、油酸、亚油酸和重水异构体的非线性磁化率的定量测量。我们发现，-D伸缩振动确实有一个较低的区域，这与原子质量从氢到氢加倍而导致的频率降低是一致的。这一发现阐明了在采用氚标记作为相干拉曼显微镜成像策略时，化学特异性和信号强度之间的一个重要权衡。氚标记是相干拉曼成像中的一个重要工具。本文报道了水、琥珀酸、油酸、亚油酸和重氢异构体的定量相干拉曼磁化率谱。对-D伸缩振动的拉曼极化率低于-H伸缩振动的理论预测进行了验证和量化。
Deuterium labelling is increasingly used in coherent Raman imaging of complex systems, such as biological cells and tissues, to improve chemical specificity. Nevertheless, quantitative coherent Raman susceptibility spectra for deuterated compounds have not been previously reported. Interestingly, it is expected theoretically that –D stretch vibrations have a Raman susceptibility lower than –H stretch vibrations, with the area of their imaginary part scaling with their wavenumber, which is shifted from around 2900 cm−1 for C–H into the silent region around 2100 cm−1 for C–D. Here, we report quantitative measurements of the nonlinear susceptibility of water, succinic acid, oleic acid, linoleic acid and deuterated isoforms. We show that the –D stretch vibration has indeed a lower area, consistent with the frequency reduction due to the doubling of atomic mass from hydrogen to deuterium. This finding elucidates an important trade‐off between chemical specificity and signal strength in the adoption of deuterium labelling as an imaging strategy for coherent Raman microscopy. Deuterium labelling is an important tool in coherent Raman imaging. Quantitative coherent Raman susceptibility spectra of water, succinic acid, oleic acid, linoleic acid and deuterated isoforms, separated into real and imaginary part, are reported. The theoretical prediction that –D stretch vibrations have a Raman susceptibility lower than –H stretch vibrations according to their reduced frequency is verified and quantified.
登录
查看更多内容
影响因子：
7.4
作者：
Masia, Francesco;Pope, Iestyn;Borri, Paola
通讯作者：
Borri, Paola
使用高光谱相干抗螺旋体拉曼散射显微镜的定量化学成像和无监督分析。
影响因子：
7.4
作者：
Masia, Francesco;Glen, Adam;Stephens, Phil;Borri, Paola;Langbein, Wolfgang
通讯作者：
Langbein, Wolfgang
DOI：
10.1039/d0an02381g
发表时间：
2021-04-07
期刊：
The Analyst
影响因子：
--
作者：
Pope I;Masia F;Ewan K;Jimenez-Pascual A;Dale TC;Siebzehnrubl FA;Borri P;Langbein W
通讯作者：
Langbein W
影响因子：
4.4
作者：
MARTIN, J;MONTERO, S
通讯作者：
MONTERO, S
影响因子：
3.8
作者：
Pope, Iestyn;Langbein, Wolfgang;Borri, Paola
通讯作者：
Borri, Paola