Consumption of miRNA-Mediated Insect-Resistant Transgenic Rice Pollen Does Not Harm Apis mellifera Adults
Consumption of miRNA-Mediated Insect-Resistant Transgenic Rice Pollen Does Not Harm Apis mellifera Adults
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食用 miRNA 介导的抗虫转基因稻花粉不会伤害意大利蜜蜂成虫
DOI：
10.1021/acs.jafc.1c00585
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发表时间：
2021-04-03
影响因子：
6.1
通讯作者：
Han, Lanzhi
中科院分区：
文献类型：
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作者：
Chen, Junjie;Wang, Huilin;Han, Lanzhi
基于RNA干扰（RNAi）的抗虫转基因（IRGE）作物通过抑制基因表达或翻译，为害虫治理提供了一种颇具前景的方法。一种已培育出的表达内源性二化螟Csu - novel - 260的微小RNA介导的IRGE水稻品系，对目标害虫表现出显著抗性。非靶标昆虫蜜蜂是一种重要的传粉者，常被用作IRGE植物生态风险评估的替代物种。为模拟最坏情况，克隆了二化螟和蜜蜂的全长离体（dib）cDNA。二化螟和蜜蜂的dib 3'-非翻译区核苷酸序列相似度为58.06%。通过生物信息学分析，未在蜜蜂的Amdib中检测到潜在的Csu - novel - 260结合位点。关于摄入Csu - novel - 260对成年蜜蜂影响的饮食RNA干扰毒性试验表明，经RNA干扰处理的蜜蜂存活率与空白对照（CK）和阴性对照（NC）处理的蜜蜂存活率相比，无显著差异。蜜蜂对Csu - novel - 260的摄取量在喂食8天后达到峰值，随后逐渐下降。Amdib的表达不受RNA干扰试验天数或处理方式的影响。这些结果表明，在饮食RNA干扰试验中，成年蜜蜂对高剂量的Csu - novel - 260不敏感，且微小RNA介导的IRGE植物对蜜蜂的影响可忽略不计。
RNAi-based insect-resistant genetically engineered (IRGE) crops represent a promising approach for pest management by suppressing gene expressions or translation. A developed microRNA-mediated IRGE rice line expressing endogenous Chilo suppressalis Csu-novel-260 shows significant resistance to target pests. The nontarget insect Apis mellifera is an important pollinator used as a surrogate species for the ecological risk assessment of IRGE plants. To simulate a worst-case scenario, the full-length C. suppressalis and A. mellifera disembodied (dib) cDNAs were cloned. The dib 3'- untranslated regions shared 58.06% nucleotide sequence similarity between C. suppressalis and A. mellifera. No potential Csu-novel-260 binding site in Amdib was detected through the bioinformatics analysis. A dietary RNAi toxicity assay of the impacts of ingested Csu-novel-260 on A. mellifera adults showed that the survival rates of RNAi-treated A. mellifera did not significantly differ from those in the blank control (CK) and negative control (NC) treatments. The Csu-novel-260 uptake by A. mellifera peaked at 8 days postfeeding and then gradually decreased. The Amdib expression was not affected by the RNAi assay days or treatments. These results suggest that A. mellifera adults are not susceptible to high doses of Csu-novel-260 in the dietary RNAi assay and that the impact of miRNA-mediated IRGE plants on A. mellifera is negligible.