Estimating the frequency of Asian cytochrome B haplotypes in standard European and local Spanish pig breeds
Estimating the frequency of Asian cytochrome B haplotypes in standard European and local Spanish pig breeds
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DOI：
10.1051/gse:2003053
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发表时间：
2004-01-01
影响因子：
4.1
通讯作者：
Sànchez, A
中科院分区：
文献类型：
--
作者：
Clop, A;Amills, M;Sànchez, A
线粒体DNA已被广泛用于不同动物物种的系统发育研究。在猪中，细胞色素B基因和D-loop区域的遗传变异已被用作分析不同品种和群体之间遗传关系的工具。在这项工作中，我们分析了细胞色素B基因上的四个SNP，以推测几个欧洲标准和地方猪品种的亚洲(A1和A2单倍型)或欧洲(E1和E2单倍型)起源。我们在加那利亚黑猪(E1，A1和A2)、德国皮特兰猪(E1，A1和A2)、比利时皮特兰猪(E1，A1)、大白猪(E1和A1)和长白猪(E1和A1)品种中发现了亚洲和欧洲单倍型的混合。相比之下，伊比利亚猪(瓜迪尔巴斯、埃尔维迪拉、卡尔代拉、坎帕纳里奥、普埃布拉和托比斯卡尔品系)和马约坎黑猪品种只表现出E1单倍型。我们的结果表明，中国猪种的导入影响了大多数主要的欧洲标准品种，这些品种具有不同频率的亚洲单倍型(15%-56%)。相比之下，孤立的西班牙本地品种，如伊比利亚和马约坎黑猪，只显示出欧洲的细胞色素B单倍型，这一特征表明它们没有与其他中国或欧洲商业种群杂交。这些发现说明了地理上的限制如何使几个当地的西班牙品种免于发生在18世纪和19世纪欧洲的大范围内倾事件。
Mitochondrial DNA has been widely used to perform phylogenetic studies in different animal species. In pigs, genetic variability at the cytochrome B gene and the D- loop region has been used as a tool to dissect the genetic relationships between different breeds and populations. In this work, we analysed four SNP at the cytochrome B gene to infer the Asian ( A1 and A2 haplotypes) or European ( E1 and E2 haplotypes) origins of several European standard and local pig breeds. We found a mixture of Asian and European haplotypes in the Canarian Black pig ( E1, A1 and A2), German Pietrain ( E1, A1 and A2), Belgian Pietrain ( E1, A1), Large White ( E1 and A1) and Landrace ( E1 and A1) breeds. In contrast, the Iberian ( Guadyerbas, Ervideira, Caldeira, Campanario, Puebla and Torbiscal strains) and the Majorcan Black pig breeds only displayed the E1 haplotype. Our results show that the introgression of Chinese pig breeds affected most of the major European standard breeds, which harbour Asian haplotypes at diverse frequencies ( 15- 56%). In contrast, isolated local Spanish breeds, such as the Iberian and Majorcan Black pig, only display European cytochrome B haplotypes, a feature that evidences that they were not crossed with other Chinese or European commercial populations. These findings illustrate how geographical confinement spared several local Spanish breeds from the extensive introgression event that took place during the 18th and 19th centuries in Europe.