Complete isolation and characterization of silybins and isosilybins from milk thistle (Silybum marianum)
Complete isolation and characterization of silybins and isosilybins from milk thistle (Silybum marianum)
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DOI：
10.1039/b300099k
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发表时间：
2003-05-21
影响因子：
3.2
通讯作者：
Wall, ME
中科院分区：
文献类型：
--
作者：
Kim, NC;Graf, TN;Wall, ME
首次采用反相高效液相色谱法从水飞蓟种子中分离、分离和结构鉴定了四种非对映异构体黄酮木脂素，即水飞蓟素A(1)和B(2)和异硅酮素A(3)和B(4)。此外，还分离到3个黄酮木脂素，分别为水飞蓟素(5)、异水飞蓟素(6)和水飞蓟素(7)，以及黄酮类化合物紫杉酯(8)。结构，包括1-4的绝对立体化学，用2D核磁共振和CD光谱进行了确认。
Complete separation, isolation, and structural characterization of four diastereoisomeric flavonolignans, silybins A (1) and B (2), and isosilybins A (3) and B (4) from the seeds of milk thistle (Silybum marianum) were achieved for the first time using a preparative reversed-phase HPLC method. In addition, three other flavonolignans, silychristin (5) isosilychristin (6) and silydianin (7), and a flavonoid, taxifolin (8) were isolated. Structures, including absolute stereochemistries of 1-4, were confirmed using 2D NMR and CD spectroscopy.