Enveloped viruses distinct from HBV induce dissemination of hepatitis D virus in vivo
Enveloped viruses distinct from HBV induce dissemination of hepatitis D virus in vivo
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DOI：
10.1038/s41467-019-10117-z
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发表时间：
2019-05-08
影响因子：
16.6
通讯作者：
Cosset, Francois-Loic
中科院分区：
文献类型：
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作者：
Perez-Vargas, Jimena;Amirache, Fouzia;Cosset, Francois-Loic
丁型肝炎病毒(HDV)不编码包装其核糖核蛋白(RNP)的包膜蛋白，通常依赖于乙肝病毒(HBV)的表面糖蛋白(GP)进行病毒粒子组装、包膜和细胞传播。HDV RNA基因组可以在不同的组织和物种中有效复制，增加了它进化和/或仍然能够传播的可能性，而不依赖于乙肝病毒。在这里，我们展示了替代的，与乙肝病毒无关的病毒可以作为HDV的辅助病毒。在体外，包括水泡病毒、黄病毒和肝炎病毒在内的几个病毒属的包膜GP可以包装HDV RNP，使HDV颗粒在共同感染的细胞外环境中有效地排出，随后进入表达相关受体的细胞。此外，丙型肝炎病毒可以在混合感染的人源化小鼠的肝脏中传播HDV感染数月。需要进一步的工作来评估HDV目前是否通过与乙肝病毒无关的病毒在人类中传播。
Hepatitis D virus (HDV) doesn't encode envelope proteins for packaging of its ribonucleoprotein (RNP) and typically relies on the surface glycoproteins (GPs) from hepatitis B virus (HBV) for virion assembly, envelopment and cellular transmission. HDV RNA genome can efficiently replicate in different tissues and species, raising the possibility that it evolved, and/or is still able to transmit, independently of HBV. Here we show that alternative, HBV-unrelated viruses can act as helper viruses for HDV. In vitro, envelope GPs from several virus genera, including vesiculovirus, flavivirus and hepacivirus, can package HDV RNPs, allowing efficient egress of HDV particles in the extracellular milieu of co-infected cells and subsequent entry into cells expressing the relevant receptors. Furthermore, HCV can propagate HDV infection in the liver of co-infected humanized mice for several months. Further work is necessary to evaluate whether HDV is currently transmitted by HBV-unrelated viruses in humans.