Population Structure and Phylogenetic Relationships in a Diverse Panel of Brassica rapa L.
Population Structure and Phylogenetic Relationships in a Diverse Panel of Brassica rapa L.
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DOI：
10.3389/fpls.2017.00321
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发表时间：
2017
影响因子：
5.6
通讯作者：
Labate JA
中科院分区：
文献类型：
--
作者：
Bird KA;An H;Gazave E;Gore MA;Pires JC;Robertson LD;Labate JA
作物品种芸苔 (Brassica rapa L.) 在世界范围内具有重要的经济重要性。然而，该物种的全球分布和复杂的进化历史使得调查其遗传种群结构变得困难。作物驯化和改良导致了极端的表型多样性和亚种，可用于油籽、人类消费的食品和牲畜饲料。这些亚种包括油籽形态型。 oleifera（芜菁油菜），ssp.萁 (brown sarson/toria), ssp.三房（黄沙森）；亚种。 rapa（萝卜）；和亚洲叶菜类蔬菜。大白菜（大白菜），亚种。 chinensis（白菜），ssp。 nipposinica (mizuna/mibuna), ssp. rapifera (rapini/西兰花拉贝), ssp. narinosa (tatsoi)、ssp parachinensis (choy sum) 和 ssp。 perviridis（科松纳）。迄今为止，研究还没有足够的样本来确定所有形态类型，特别是油籽形态类型的关系，并且对于形态类型和地理起源对种群结构的贡献仍然存在疑问。我们使用测序基因分型对全球多样化的 333 个白菜国家植物种质系统种质（包括 10 个公认的亚种）中的 18,272 个单核苷酸多态性标记进行了评分。我们的群体遗传和系统发育分析大致一致，并揭示了五个亚群体，这些亚群体在很大程度上反映了形态类型和地理。这些亚群是 1. 欧洲萝卜/油籽、2. 亚洲萝卜/油籽、3. 黄/棕沙森 (ssp. trilularis 和 ssp. dichotoma)、4. 大白菜 (ssp. pekinensis) 和 5. 白菜、菜心和 tatsoi (ssp. chinensis、ssp. parachinensis、纳里诺萨亚种）。此外，我们还发现了多系和/或多系的证据，特别是油籽形态型（油籽亚种和萁亚种）和芜菁。这项研究的结果为白菜物种内亚种的遗传和系统发育关系提供了更好的解决方案。了解这些关系对于未来遗传研究和这一全球重要作物品种的改良至关重要。
The crop species Brassica rapa L. has significant economic importance around the world. However, the global distribution and complex evolutionary history of the species has made investigating its genetic population structure difficult. Crop domestication and improvement has resulted in extreme phenotypic diversity and subspecies that are used for oilseed, food for human consumption, and fodder for livestock. These subspecies include the oilseed morphotypes. oleifera (turnip rape), ssp. dichotoma (brown sarson/toria), ssp. trilocularis (yellow sarson); ssp. rapa (turnip); and Asian leafy vegetables ssp. pekinensis (Chinese cabbage), ssp. chinensis (bok choy), ssp. nipposinica (mizuna/mibuna), ssp. rapifera (rapini/broccoli rabe), ssp. narinosa (tatsoi), ssp parachinensis (choy sum), and ssp. perviridis (komatsuna). To date, studies have had insufficient sampling to determine the relationship of all morphotypes, especially oilseed morphotypes, and questions remain over the contribution of morphotype and geographic origin to population structure. We used genotyping-by-sequencing to score 18,272 single nucleotide polymorphism markers in a globally diverse panel of 333 B. rapa National Plant Germplasm System accessions that included 10 recognized subspecies. Our population genetic and phylogenetic analyses were broadly congruent and revealed five subpopulations that were largely reflective of morphotype and geography. These subpopulations were 1. European turnips/oilseed, 2. Asian turnips/oilseed, 3. yellow/brown sarson (ssp. trilocularis and ssp. dichotoma), 4. Chinese cabbage (ssp. pekinensis), and 5. bok choy, choy sum, and tatsoi (ssp. chinensis, ssp. parachinensis, ssp. narinosa). Additionally, we found evidence of polyphyly and/or paraphyly, particularly for oilseed morphotypes (ssp. oleifera and ssp. dichotoma) and turnips. The results of this study have provided improved resolution to the genetic and phylogenetic relationships of subspecies within the species B. rapa. Understanding of these relationships is key to the future genetic study and improvement of this globally important crop species.