Electrophysiological Studies Revealed CaM1-Mediated Regulation of the Arabidopsis Calcium Channel CNGC12
Electrophysiological Studies Revealed CaM1-Mediated Regulation of the Arabidopsis Calcium Channel CNGC12
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电生理学研究揭示 CaM1 介导的拟南芥钙通道 CNGC12 调节
DOI：
10.3389/fpls.2019.01090
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发表时间：
2019-09
影响因子：
5.6
通讯作者：
Zhu Huifen
中科院分区：
文献类型：
--
作者：
Zhang Zhengli;Hou Congcong;Tian Wang;Li Legong;Zhu Huifen
拟南芥环核苷酸门控通道（CNGC）家族由20个成员组成，广泛参与病原菌防御、发育和耐热等生理过程。尽管CNGC 11和CNGC 12在十年前就已被鉴定，并且它们在程序性细胞死亡中的作用已被充分研究，但它们的精确通道调节尚未被电生理学研究。在此，我们利用双电极电压钳技术在非洲爪蟾卵母细胞异源表达系统中测定了CNGC 11和CNGC 12的通道活性。我们的研究结果表明，CNGC 12，而不是CNGC 11作为一个活跃的钙通道的功能。此外，环核苷酸单磷酸（cNMPs）不影响CNGC 11或CNGC 12在爪蟾卵母细胞中的活性。有趣的是，虽然CNGC 11的活性不受与钙调素（CaM）共表达的影响，但当CaM 1在卵母细胞中共表达时，CNGC 12的活性显著增强。本研究表明CNGC 11和CNGC 12的通道活性和CaM的调节机制不同
The Arabidopsis cyclic nucleotide-gated channel (CNGC) family consists of 20 members, which have been reported to participate in various physiological processes, such as pathogen defense, development, and thermotolerance. Although CNGC11 and CNGC12 have been identified a decade ago and their role in programmed cell death is well studied, their precise channel regulation has not been studied electrophysiologically. Here, we determined the channel activities of CNGC11 and CNGC12 utilizing the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. Our results suggest that CNGC12 but not CNGC11 functions as an active calcium channel. Furthermore, the cyclic nucleotide monophosphates (cNMPs) did not affect the activities of CNGC11 nor CNGC12 in Xenopus oocytes. Interestingly, while the activity of CNGC11 was not affected by co-expression with calmodulin (CaM), the activity of CNGC12 was significantly enhanced when CaM1 was co-expressed in oocytes. This study reveals that the channel activities and the mechanisms of regulation by CaM are different between CNGC11 and CNGC12
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影响因子：
7.4
作者：
Leng, Q;Mercier, RW;Berkowitz, GA
通讯作者：
Berkowitz, GA
影响因子：
27.5
作者：
Liming Zhou;Wenzhi Lan;Yuan-Qing Jiang;Wei-Ling Fang;S. Luan
通讯作者：
Liming Zhou;Wenzhi Lan;Yuan-Qing Jiang;Wei-Ling Fang;S. Luan
DOI：
10.1007/978-1-60327-317-6_25
发表时间：
2009-01-01
期刊：
G PROTEIN-COUPLED RECEPTORS IN DRUG DISCOVERY
影响因子：
--
作者：
Hansen, Kasper B.;Brauner-Osborne, Hans
通讯作者：
Brauner-Osborne, Hans
拟南芥 CNGC14 介导根毛尖端生长所需的钙流入
影响因子：
27.5
作者：
Zhang Sisi;Pan Yajun;Dong Mengqi;Zhu Huifen;Li Legong;Zhu Huifen;Li Legong;Tian Wang;Luan Sheng;Li LG;Luan S
通讯作者：
Luan S
影响因子：
11.6
作者：
Ali, Rashid;Ma, Wei;Berkowitz, Gerald A.
通讯作者：
Berkowitz, Gerald A.