Rapid localization of Gag/GagPol complexes to detergent-resistant membrane during the assembly of human immunodeficiency virus type 1
Rapid localization of Gag/GagPol complexes to detergent-resistant membrane during the assembly of human immunodeficiency virus type 1
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DOI：
10.1128/jvi.77.7.3973-3984.2003
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发表时间：
2003-04-01
影响因子：
5.4
通讯作者：
Kleiman, L
中科院分区：
文献类型：
--
作者：
Halwani, R;Khorchid, A;Kleiman, L
在人类免疫缺陷病毒1型（HIV-1）的装配在HIV-1转染的COS 7细胞，几乎所有的稳态Gag/Gag和Gag/GagPol复合物的膜结合。然而，暴露于1%Triton X-100得到的结果表明，虽然所有的Gag/GagPol复合物保持与耐洗涤剂膜（DRM）相关联，但只有30%的Gag/Gag复合物与DRM相关联。新合成的Gag/Gag和Gag/GagPol在膜上的定位分析表明，在用放射性[S-35]Cys-[S-35] Met脉冲10分钟后，在DRM处发现了所有新合成的Gag/GagPol。只有30%的新合成的Gag/Gag移动到膜上，并且在追踪0分钟时，只有38%的这种膜结合的Gag/Gag与DRM相关。在追逐的前30分钟期间，大多数膜结合的Gag/Gag移动到DRM，而在追逐的30和60分钟之间，膜结合的Gag/Gag和Gag/GagPol显著降低。由于新合成的Gag/Gag向DRM的定位以及GagPol与Gag的相互作用都依赖于Gag多聚化，因此GagPol向DRM的快速定位可能反映了所有新合成的GagPol与第一个新合成的聚合Gag的相互作用以与DRM缔合。
During human immunodeficiency virus type 1 (HIV-1) assembly in HIV-1-transfected COS7 cells, almost all steady-state Gag/Gag and Gag/GagPol complexes are membrane bound. However, exposure to 1% Triton X-100 gives results indicating that while all Gag/GagPol complexes remain associated with the detergent-resistant membrane (DRM), only 30% of Gag/Gag complexes are associated with the DRM. Analysis of the localization of newly synthesized Gag/Gag and Gag/GagPol to the membrane indicates that after a 10-min pulse with radioactive [S-35]Cys-[S-35] Met, all newly synthesized Gag/GagPol is found at the DRM. Only 30% of newly synthesized Gag/Gag moves to the membrane, and at 0 min of chase, only 38% of this membrane-bound Gag/Gag is associated with the DRM. During the first 30 min of chase, most membrane-bound Gag/Gag moves to the DRM, while between 30 and 60 min of chase, there is a significant decrease in membrane-bound Gag/Gag and Gag/GagPol. Since the localization of newly synthesized Gag/Gag to the DRM and the interaction of GagPol with Gag both depend upon Gag multimerization, the rapid localization of GagPol to the DRM probably reflects the interaction of all newly synthesized GagPol with the first newly synthesized polymeric Gag to associate with the DRM.