In situ hybridization evidence for the coexistence of ASIC and TRPV1 within rat single sensory neurons
In situ hybridization evidence for the coexistence of ASIC and TRPV1 within rat single sensory neurons
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DOI：
10.1016/j.molbrainres.2005.01.010
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发表时间：
2005-05-20
期刊：
影响因子：
--
通讯作者：
Shimada, S
中科院分区：
文献类型：
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作者：
Ugawa, S;Ueda, T;Shimada, S
质子对伤害感受器的激活在酸中毒相关疼痛的起始和维持中起着至关重要的作用。酸敏感离子通道（ASIC）和瞬时受体电位/香草素受体亚型1（TRPV 1）编码由伤害感受器表达的质子激活阳离子通道，这些通道的开放导致伤害感受器兴奋。在大鼠背根神经节（DRG）连续切片上，采用原位杂交组织化学方法研究了ASIC克隆之间的组织学关系以及单个感觉神经元内每个ASIC亚基和TRPV 1的共定位。ASIC 1a转录本在20-25%的DRG神经元中表达，并且大多数神经元具有小（< 30 μ m）直径的细胞体。ASIC 1b转录本和ASIC 3转录本分别在10%和30-35%的DRG神经元中表达，并且每个群体的大部分位于小到中等（30-50 μ m）直径的细胞中。ASIC 1a和ASIC 1b转录本基本上定位于DRG神经元的不同群体中，而ASIC 1a阳性神经元的20%和ASIC 1b阳性神经元的10%表达ASIC 3转录本。TRPV 1转录本在35-40%的DRG神经元中表达，并且大多数TRPV 1阳性神经元具有小直径的细胞体。在40-45%的TRPV 1阳性神经元中检测到ASIC 1a转录物的强烈表达信号。神经元表达ASIC 1b和TRPV 1转录几乎没有检测到的DRG。大约30%的TRPV 1阳性神经元表达ASIC 3转录本，双标记神经元由小直径和中等直径的细胞组成。大约13%的TRPV 1阳性神经元同时表达ASIC 1a和ASIC 3转录本。(c)2005 Elsevier B. V.保留所有权利。
The activation of nociceptors by protons plays a crucial role in the initiation and maintenance of acidosis-linked pain. Acid-sensing ion channel (ASIC) and transient receptor potentia/vanilloid receptor subtype-1 (TRPV1) encode proton-activated cation channels expressed by nociceptors and the opening of these channels results in nociceptor excitation. Histological relations among ASIC clones and the colocalization of each ASIC subunit and TRPV1 within single sensory neurons were examined on serial sections of rat dorsal root ganglia (DRG) using in situ hybridization histochemistry. ASIC1a transcripts were expressed in 20-25% of the DRG neurons, and most of the neurons had small (< 30 mu m)-diameter cell bodies. ASIC1b transcripts and ASIC3 transcripts were expressed in similar to 10% and 30-35% of the DRG neurons, respectively, and the greater part of each population was located in small-to-medium (30-50 pm)-diameter cells. The ASIC I a transcripts and ASIC1b transcripts were basically localized in the distinct populations of the DRG neurons, while similar to 20% of the ASIC1a-positive neurons and similar to 10% of the ASIC1b-positive neurons expressed ASIC3 transcripts. TRPV1 transcripts were expressed in 35-40% of the DRG neurons, and most of the TRPV1-positive neurons had small-diameter cell bodies. Intense expression signals for ASIC1a transcripts were detected in 40-45% of the TRPV1-positive neurons. Neurons expressing both ASIC1b and TRPV1 transcripts were barely detected in the DRG. Approximately 30% of the TRPV1-positive neurons expressed ASIC3 transcripts, and the double-labeled neurons were comprised of both small-diameter and medium-diameter cells. Approximately 13% of the TRPV1-positive neurons expressed both ASIC1a and ASIC3 transcripts. (c) 2005 Elsevier B.V. All rights reserved.